Muller glia are a potential source of neural regeneration in the postnatal chicken retina
Muller glia are a potential source of neural regeneration in the postnatal chicken retina
复制标题
DOI：
10.1038/85090
复制
发表时间：
2001-03-01
影响因子：
25
通讯作者：
Reh, TA
中科院分区：
文献类型：
--
作者：
Fischer, AJ;Reh, TA
温血脊椎动物的视网膜被认为没有神经再生的能力。在这里，我们提供了出生后鸡的视网膜具有产生新神经元的潜力的证据。作为对急性损伤的反应，许多Muller胶质细胞重新进入细胞周期，此后不久，表达了由胚胎视网膜前体细胞表达的转录因子Cash-1、Pax6和Chx10。这些祖细胞样细胞瞬时表达神经丝。新形成的细胞分布在视网膜的内外核层，并在损伤后至少保持三周。这些新形成的细胞部分分化为视网膜神经元，少数形成Muller胶质细胞，大部分仍未分化，Pax6和Chx10继续表达。这些细胞在培养中继续增殖，其中一些分化为视网膜神经元或穆勒胶质细胞。我们认为，作为对损伤的反应，视网膜中的穆勒胶质细胞是神经再生的潜在来源。
The retina of warm-blooded vertebrates is believed to be incapable of neural regeneration. Here we provide evidence that the retina of postnatal chickens has the potential to generate new neurons. In response to acute damage, numerous Muller glia re-entered the cell cycle, and shortly thereafter, expressed CASH-1, Pax6 and Chx10, transcription factors expressed by embryonic retinal progenitors. These progenitor-like cells transiently expressed neurofilament. Newly formed cells became distributed throughout the inner and outer nuclear layers of the retina, and remained for at least three weeks after damage. Some of these newly formed cells differentiated into retinal neurons, a few formed Muller glia, and most remained undifferentiated, with continued expression of Pax6 and Chx10. These cells continued to proliferate when grown in culture, with some differentiating into retinal neurons or Muller glia. We propose that, in response to damage, Muller glia in the retina are a potential source of neural regeneration.