Norovirus pathway in water environment estimated by genetic analysis of strains from patients of gastroenteritis, sewage, treated wastewater, river water and oysters
Norovirus pathway in water environment estimated by genetic analysis of strains from patients of gastroenteritis, sewage, treated wastewater, river water and oysters
复制标题
DOI：
10.1016/j.watres.2005.06.035
复制
发表时间：
2005-11-01
期刊：
影响因子：
12.8
通讯作者：
Omura, T
中科院分区：
文献类型：
--
作者：
Ueki, Y;Sano, D;Omura, T
在本研究中，诺如病毒（NV）衣壳基因检测胃肠炎患者，生活污水，污水处理厂，河水和养殖牡蛎在地理上接近的地区，所有的样品收集。为了提高牡蛎中NVs的回收效率，开发了一种新的方法，即使用喷雾装置。结果表明，2003年11月至2004年2月，在30只牡蛎中，有18只（60%）呈NV基因阳性，而在同一采样点的30只牡蛎中，有7只（23%）呈NV基因阳性。这些结果表明，我们的新方法表现出更高的效率，回收NVs比传统的超离心方法。2003年11月至2004年2月，8份河水中有6份（75%）NV基因阳性。9份处理后的污水中有8份（89%）NV基因阳性，同期9份生活污水均为NV基因阳性。这些结果表明，处理后的废水将是该地区NV污染的主要来源之一。对分离到的NV衣壳蛋白基因进行了同源性分析，发现来自患者、生活污水、河水和养殖牡蛎的NV衣壳蛋白基因序列具有高度的同源性。这些结果表明，在人类和养殖牡蛎之间可能存在地理关联的NVs通过水环境循环。因此，定量分析NV的迁移途径，直接关系到水环境和养殖牡蛎NV污染防治新方案的制定。(c)2005爱思唯尔有限公司保留所有权利。
In this study, Norovirus (NV) capsid gene was detected from patients of gastroenteritis, domestic sewage, treated wastewater, river water and cultivated oysters in geographically close areas where all of samples were collected. In order to improve recovery efficiency of NVs from oysters, a new method using a spallation apparatus was developed. As a result, 18 of 30 oysters (60%) were positive for NV gene, while 7 of 30 (23%) oysters from the same sampling point were positive with the conventional ultracentrifugal method between November 2003 and February 2004. These results indicate that our new method exhibits the higher efficiency of recovering NVs than the conventional ultracentrifugal method. Six of 8 samples (75%) of river water were positive for NV gene between November 2003 and February 2004. Further-more, 8 of 9 samples (89%) of treated wastewater and all 9 samples of sewage were positive for NV gene in the same period. These results indicated that treated wastewater would be one of the main sources for NV pollution in this area. The phylogenctic analysis in isolated NV capsid genes was conducted, in which high identities of gene sequences between NVs from patients, domestic sewage, river water and cultivated oysters were observed. These results implied that there would be a geographically associated circulation of NVs between human and cultivated oysters via water environment. It would be important to quantitatively analyze the moving pathway of NVs, which directly link to the development of a new scheme for preventing water environment and cultivated oysters from NV contamination. (c) 2005 Elsevier Ltd. All rights reserved.