Phosphorylation and dephosphorylation of threonine 188 in nucleoprotein is crucial for the replication of influenza A virus
Phosphorylation and dephosphorylation of threonine 188 in nucleoprotein is crucial for the replication of influenza A virus
复制标题
核蛋白中苏氨酸188的磷酸化和去磷酸化对于甲型流感病毒的复制至关重要
DOI：
10.1016/j.virol.2018.05.002
复制
发表时间：
2018-07-01
期刊：
影响因子：
3.7
通讯作者：
Luo, Ting Rong
中科院分区：
文献类型：
--
作者：
Li, Yun;Sun, Lei;Luo, Ting Rong
Nucleoprotein (NP) is a major component of the viral ribonucleoprotein (vRNP) complex that is responsible for viral replication, transcription and packaging of influenza A virus. Phosphorylation of NP plays an important role during viral infection. In the present study, we identified threonine 188 (T188) as a novel phosphorylated residue in the NP of influenza A virus by using mass spectrometry. T188 is located within nuclear export signal 2 (NES2) which is chromosome region maintenance 1 (CRM1)-independent. We observed that the phosphorylation and dephosphorylation of residue T188 regulated viral replication by controlling NES2-dependent NP nuclear export and the polymerase activity of the vRNP complex. Our findings provide further insights for understanding the replication of influenza A virus.
