Enzyme-like immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms.
Enzyme-like immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms.
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针对昆虫毒液的免疫球蛋白 G 抗体的酶样免疫吸附测定 (ELISA)。
DOI：
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发表时间：
1981
影响因子：
14.2
通讯作者：
J. Gillaspy
中科院分区：
文献类型：
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作者：
J. Grant;R. Goldblum;R. Rahr;D. Thueson;J. Farnam;J. Gillaspy
在接受虫毒免疫治疗的患者中检测IgG“阻断”抗体。本文所描述的酶联免疫吸附测定（ELISA）是敏感和可重复性的。ELISA结果与放射免疫测定结果以及抑制敏感嗜碱性细胞组胺释放的结果具有良好的相关性。此外，抗磷脂酶A和全蜂毒的特异性抗体滴度也有相关性。对17例接受Polistes黄蜂或蜂毒免疫治疗的患者进行了血清特异性IgG抗体的系列检测。大多数患者显示出IgG抗体的上升，在服用约500微克毒液后达到峰值。在13名接受毒液治疗的患者中，只有1人在接受维持治疗时被昆虫叮咬后出现过敏症状。
IgG "blocking" antibodies were measured in patients receiving insect venom immunotherapy. The enzyme-linked immunosorbent assay (ELISA) described herein was found to be sensitive and reproducible. Results with ELISA correlated well with values obtained with a radioimmunoassay and with inhibition of the release of histamine from sensitive basophils. Also, specific antibody titers against phospholipase A and whole bee venom were correlated. Serial determinations of venom-specific IgG antibodies were made in 17 patients receiving Polistes wasp or bee venom immunotherapy. The majority of patients showed a rise in IgG antibodies, which peaked after administration of approximately 500 micrograms of venom. Only one out of 13 of these venom-treated patients had allergic symptoms after an insect sting while on maintenance therapy.