Effects of cerebral ischemia on neuronal hemoglobin.
Effects of cerebral ischemia on neuronal hemoglobin.
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DOI：
10.1038/jcbfm.2008.145
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发表时间：
2009-03
期刊：
影响因子：
--
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中科院分区：
文献类型：
--
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本研究探讨是否存在神经元血红蛋白（Hb）在大鼠。然后研究脑缺血或缺血预处理（IPC）是否影响体内和体外神经元Hb水平。在体内，使雄性Sprague-Dawley大鼠经受15分钟的短暂大脑中动脉闭塞伴24小时再灌注、IPC刺激、或24小时的永久大脑中动脉闭塞（pMCAO）、或IPC三天后接着24小时的pMCAO。在体外，原代培养的神经元暴露于2小时的氧-葡萄糖剥夺和22小时的复氧。结果表明，Hb在大鼠脑神经元中广泛表达，而在星形胶质细胞中不表达。IPC后，同侧尾状核和大脑中动脉区皮质核心Hb表达显著上调。pMCAO后24小时，更多半影区皮质和对侧半球的Hb水平也增加，但同侧尾状核和皮质核心区的表达减少。缺血预处理改善pMCAO诱导的脑Hb变化。体外神经元血红蛋白水平增加2小时的氧-糖剥夺和22小时的复氧。这些结果表明，Hb是在神经元中合成的，并且可以通过缺血上调。
The present study examined whether or not neuronal hemoglobin (Hb) is present in rats. It then examined whether cerebral ischemia or ischemic preconditioning (IPC) affects neuronal Hb levels in vivo and in vitro. In vivo, male Sprague-Dawley rats were subjected to either 15 minutes of transient middle cerebral artery occlusion with 24 hours of reperfusion, an IPC stimulus, or 24 hours of permanent middle cerebral artery occlusion (pMCAO), or IPC followed three days later by 24 hours of pMCAO. In vitro, primary cultured neurons were exposed to 2 hours of oxygen-glucose deprivation with 22 hours of reoxygenation. Results showed that Hb is widely expressed in rat cerebral neurons but not astrocytes. Hb expression was significantly upregulated in the ipsilateral caudate and the cortical core of the middle cerebral artery territory after IPC. Hb levels also increased in more penumbral cortex and the contralateral hemisphere 24 hours after pMCAO, but expression in the ipsilateral caudate and cortical core area were decreased. Ischemic preconditioning modified pMCAO-induced brain Hb changes. Neuronal Hb levels in vitro were increased by 2 hours of oxygen-glucose deprivation and 22 hours of reoxygenation. These results indicate that Hb is synthesized in neurons and can be upregulated by ischemia.
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DOI：
10.1083/jcb.85.3.890
发表时间：
1980-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
McCarthy KD;de Vellis J
通讯作者：
de Vellis J
DOI：
10.1073/pnas.0607497103
发表时间：
2006-11-21
影响因子：
11.1
作者：
Khan, Adil A.;Wang, Yaoming;Greenberg, David A.
通讯作者：
Greenberg, David A.
DOI：
10.1073/pnas.0637726100
发表时间：
2003-03-18
影响因子：
11.1
作者：
Sun, YJ;Jin, KL;Greenberg, DA
通讯作者：
Greenberg, DA
DOI：
10.1097/01.wcb.0000091762.61714.fe
发表时间：
2003-11-01
影响因子：
6.3
作者：
Andjelkovic, AV;Stamatovic, SM;Keep, RF
通讯作者：
Keep, RF
影响因子：
37.8
作者：
MURRY, CE;JENNINGS, RB;REIMER, KA
通讯作者：
REIMER, KA