A global definition of expression context is conserved between orthologs, but does not correlate with sequence conservation.
A global definition of expression context is conserved between orthologs, but does not correlate with sequence conservation.
复制标题
直系同源物之间的表达上下文的全局定义是保守的，但与序列保守无关。
DOI：
10.1186/1471-2164-7-10
复制
发表时间：
2006-01-19
期刊：
影响因子：
4.4
通讯作者：
Snel, Berend
中科院分区：
文献类型：
--
作者：
Dutilh, Bas E;Huynen, Martijn A;Snel, Berend
大规模的微阵列衍生基因表达数据允许细胞功能的全局视图。迄今为止，物种间基因表达的比较研究都是基于该基因在相应组织中的表达水平，或基于该基因与另一基因的共表达。为了在全球范围内比较远缘物种之间的基因表达，我们引入了“表达背景”。一个基因的表达上下文是基于在两个基因组中具有明确对应的所有其他基因的共表达。利用这种新的测量方法，我们发现1)表达上下文在同源物之间很大程度上是保守的，2)序列同一性在基因复制和物种形成后与表达上下文保守的相关性很小。这意味着序列同一性的程度对同源物之间的差异表达上下文保护具有有限的预测质量，因此也可能是基因功能的其他方面。
The massive scale of microarray derived gene expression data allows for a global view of cellular function. Thus far, comparative studies of gene expression between species have been based on the level of expression of the gene across corresponding tissues, or on the co-expression of the gene with another gene. To compare gene expression between distant species on a global scale, we introduce the "expression context". The expression context of a gene is based on the co-expression with all other genes that have unambiguous counterparts in both genomes. Employing this new measure, we show 1) that the expression context is largely conserved between orthologs, and 2) that sequence identity shows little correlation with expression context conservation after gene duplication and speciation. This means that the degree of sequence identity has a limited predictive quality for differential expression context conservation between orthologs, and thus presumably also for other facets of gene function.