Maturational differences in superficial and deep zone articular chondrocytes
Maturational differences in superficial and deep zone articular chondrocytes
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DOI：
10.1007/s00441-005-0050-y
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发表时间：
2006-01-01
影响因子：
3.6
通讯作者：
Torzilli, PA
中科院分区：
文献类型：
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作者：
Hidaka, C;Cheng, C;Torzilli, PA
为了研究骨骼未成熟个体与成年个体的软骨细胞差异是否对软骨愈合、修复或组织工程有重要意义，我们从未成熟（1-4个月）和年轻成年（18-36个月）的牡牛身上采集了浅层软骨细胞（SZC，取自关节表面100 μ m内）和深层软骨细胞（DZC，取自关节软骨最深处未矿化部分的30%-45%），并进行了比较。细胞大小、基质基因表达和蛋白水平、整合素水平和趋化能力在微质量培养中维持7天。无论年龄大小，SZC体型较小，II型与I型胶原基因表达比较低，而SZ蛋白的基因表达量高于DZC。无论哪个区，来自未成熟牛的软骨细胞具有较高水平的Sox 9和II型胶原基因表达。培养7 d后，未成熟阉牛SZC的增殖率最高。在表型上，未成熟和成年阉牛的SZC比各自的DZC更稳定。未成熟母猪SZC细胞表面α 5和α 2整合素亚基水平高于成年母猪，而β 1整合素亚基水平相似。未成熟和成年SZC对胎牛血清和碱性成纤维细胞生长因子均具有趋化性。我们的数据表明，关节软骨细胞在软骨的不同区域和随着供体的年龄而变化。这些差异对于软骨生长、组织工程和/或修复可能是重要的。
To examine whether differences in chondrocytes from skeletally immature versus adult individuals are important in cartilage healing, repair, or tissue engineering, superficial zone chondrocytes (SZC, from within 100 mu m of the articular surface) and deep zone chondrocytes (DZC, from 30%-45% of the deepest un-mineralized part of articular cartilage) were harvested from immature (1-4 months) and young adult (18-36 months) steers and compared. Cell size, matrix gene expression and protein levels, integrin levels, and chemotactic ability were measured in cells maintained in micromass culture for up to 7 days. Regardless of age, SZC were smaller, had a lower type II to type I collagen gene expression ratio, and higher gene expression of SZ proteins than their DZC counterparts. Regardless of zone, chondrocytes from immature steers had higher levels of Sox 9 and type II collagen gene expression. Over 7 days in culture, the SZC of immature steers had the highest rate of proliferation. Phenotypically, the SZC of immature and adult steers were more stable than their respective DZC. Cell surface alpha 5 and alpha 2 integrin subunit levels were higher in the SZC of immature than of adult steers, whereas beta 1 integrin subunit levels were similar. Both immature and adult SZC were capable of chemotaxis in response to fetal bovine serum or basic fibroblast growth factor. Our data indicate that articular chondrocytes vary in the different zones of cartilage and with the age of the donor. These differences may be important for cartilage growth, tissue engineering, and/or repair.