Iron sources on iron status and gene expression of iron related transporters in iron-deficient piglets
Iron sources on iron status and gene expression of iron related transporters in iron-deficient piglets
复制标题
铁源对缺铁仔猪铁状态和铁相关转运蛋白基因表达的影响
DOI：
10.1016/j.anifeedsci.2013.03.005
复制
发表时间：
2013-06-12
影响因子：
3.2
通讯作者：
Feng, J.
中科院分区：
文献类型：
--
作者：
Fang, C. L.;Zhuo, Z.;Feng, J.
该研究旨在比较硫酸亚铁 (FeSO4) 和甘氨酸铁螯合物 (Fe-Gly) 对缺铁仔猪中铁状态和铁相关转运蛋白基因表达的影响。选择 18 头仔猪（杜洛克 x 长白猪 x 约克夏猪；6.50 +/- 0.52 公斤）进行研究。在第 1 阶段，所有猪均饲喂缺铁日粮（基础日粮；20.23 mg Fe/kg 日粮）19 天，直至诱发缺铁性贫血。在第 2 阶段，18 头仔猪被随机分配到 3 种饮食治疗，持续 10 天。饮食治疗如下：（1）对照组（基础饮食）； (2)FeSO4组(基础饲料+铁120mg/kg，以FeSO4计)； (3)Fe-Gly组(基础饮食+120mg/kg铁作为Fe-Gly)。经过10 d饲喂试验，饲喂Fe-Gly或FeSO4的仔猪心、肝、肺铁含量较高（P < 0.05），而血清总铁结合力（TIBC）较低。 (P < 0.05) 高于缺铁仔猪。与饲喂 FeSO4 的仔猪相比，饲喂 Fe-Gly 的仔猪十二指肠和空肠中肽转运蛋白 1 (PepT1) mRNA 水平较高（P < 0.05），而铁转运蛋白 1 mRNA 水平较低（P < 0.05）。然而，仔猪的生长性能在所有处理之间没有差异（P > 0.05）。总之，目前的研究表明，Fe-Gly 在改善缺铁仔猪铁状况方面比 FeSO4 更有效。此外，FeSO4 组和 Fe-Gly 组之间铁相关转运蛋白的基因表达也不同。结果还表明 PepT1 可能在 Fe-Gly 的吸收中发挥至关重要的作用。 (c) 2013 Elsevier B.V. 保留所有权利。
The study was conducted to compare the effects of ferrous sulfate (FeSO4) and iron glycine chelate (Fe-Gly) on iron status and gene expression of iron related transporters in iron-deficient piglets. Eighteen piglets (Duroc x Landrace x Yorkshire; 6.50 +/- 0.52 kg) were selected to conduct the study. In period 1, all pigs were fed an iron-deficient diet (basal diet; 20.23 mg Fe/kg of diet) for 19 days until iron-deficient anemia was induced. In period 2, the eighteen piglets were randomly allotted to 3 dietary treatments for another 10 days. Dietary treatments were as follows: (1) control group (basal diet); (2) FeSO4 group (basal diet + 120 mg/kg of iron as FeSO4); (3) Fe-Gly group (basal diet + 120 mg/kg of iron as Fe-Gly). After 10-d feeding trial, piglets fed Fe-Gly or FeSO4 had higher (P < 0.05) iron content in heart, liver and lung, whereas serum total iron binding capacity (TIBC) was lower. (P < 0.05) than iron-deficient piglets. Piglets fed Fe-Gly had higher (P < 0.05) peptide transporter 1 (PepT1) mRNA level, lower (P < 0.05) ferroportin1 mRNA level both in duodenum and jejunum when compared with pigs fed FeSO4. However, growth performance of piglets did not differ among all treatments (P > 0.05). In summary, current study showed that Fe-Gly was more effective in improving iron status of iron-deficient piglets than FeSO4. Moreover, gene expression of iron related transporters differ between FeSO4 and Fe-Gly group. Results also suggest that PepT1 may play a crucial role in absorption of Fe-Gly. (c) 2013 Elsevier B.V. All rights reserved.