Location of dye-coupled second order and of efferent vestibular neurons labeled from individual semicircular canal or otolith organs in the frog
Location of dye-coupled second order and of efferent vestibular neurons labeled from individual semicircular canal or otolith organs in the frog
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DOI：
10.1016/s0006-8993(01)03075-x
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发表时间：
2001-12-07
期刊：
影响因子：
2.9
通讯作者：
Dieringer, N
中科院分区：
文献类型：
--
作者：
Birinyi, A;Straka, H;Dieringer, N
用生物胞素选择性地标记了支配蛙三个半规管或三个耳石器官感觉上皮的前庭神经分支。标记的传入纤维半规管，椭圆囊，和lagena遇到的四个前庭核和它们的预测重叠相当大。囊状传入纤维投射到背（声）核和较小的预测前庭核的区域限制。每半规管或耳石器官约相等数量（11-14）的中等大小的前庭神经元（直径在7.5和17妈妈之间）染料耦合到传入纤维。这些染色偶联前庭神经元大多位于VIIIth和IX神经之间的前庭外侧核和前庭降核。上级前庭神经核是相对自由的染料耦合前庭神经元。中枢前庭神经元的这个亚群的位置支持了这些神经元是特定前庭脊髓通路的一部分的概念。另外。从每个耳道和/或耳石器官逆行标记约3-4个传出前庭神经元。这些神经元（直径在15和26 μ m之间）位于前庭核复合体的腹侧。传出前庭神经元的分支显示的神经元的存在下，被两种不同的荧光染料在同一实验中应用到相同的第VIII神经的前支和后支，分别双标记。这些传出神经元轴突的分支解释了一些未经处理的同侧终末器官的感觉上皮中存在的侧枝和末梢。(C)2001 Elsevier Science B. V.保留所有权利。
Vestibular nerve branches innervating the sensory epithelia of the three semicircular canals or of the three otolith organs of frogs were selectively labeled in-vitro with biocytin. Labeled afferent fibers from the semicircular canals, utricle, and lagena were encountered in each of the four vestibular nuclei and their projections overlapped considerably. Saccular afferent fibers projected to the dorsal (acoustic) nuclei and smaller projections to the vestibular nuclei were regionally restricted. Per semicircular canal or otolith organ about equal numbers (11-14) of medium sized vestibular neurons (between 7.5 and 17 mum in diameter) were dye-coupled to afferent fibers. Most of these dye-coupled vestibular neurons were located in the lateral and descending vestibular nuclei between the VIIlth and IXth nerves. The superior vestibular nucleus was relatively free of dye-coupled vestibular neurons. The location of this subpopulation of central vestibular neurons supports the notion that these neurons are part of a particular vestibulospinal pathway. In addition. from each of the canal and/or otolith organs about 3-4 efferent vestibular neurons were labeled retrogradely. These neurons (between 15 and 26 mum in diameter) were located ventral to the vestibular nuclear complex. The branching of efferent vestibular neurons was shown by the presence of neurons that were double labeled by two different fluorescent dyes applied in the same experiment to the anterior and posterior ramus of the same VIIIth nerve, respectively. The branching of these efferent neuron axons explained the presence of collaterals and terminals in the sensory epithelia of a number of untreated ipsilateral endorgans. (C) 2001 Elsevier Science B.V. All rights reserved.