Effects of recombinant human G-CSF and GM-CSF on primary human leukemic cells.
Effects of recombinant human G-CSF and GM-CSF on primary human leukemic cells.
复制标题
重组人 G-CSF 和 GM-CSF 对原代人白血病细胞的影响。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
K. Hirashima
中科院分区：
文献类型：
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作者：
K. Itoh;M. Bessho;K. Hirashima
研究了重组人粒细胞集落刺激因子（rhG-CSF）和重组人粒细胞-巨噬细胞集落刺激因子（rhGM-CSF）对人白血病原代细胞的作用。从11例AML患者和2例ALL患者的外周血和2例ALL患者的骨髓抽吸液中获得了含有88%以上原细胞的吞噬细胞耗尽的单核细胞。白血病细胞与这些脑脊液在悬浮培养或甲基纤维素培养中孵育。在悬浮培养中，1例M4患者观察到自发增殖。RhG-CSF在5例AML中刺激白血病细胞增殖，在4例AML中刺激白血病细胞增殖。在甲基纤维素培养中，3例M4患者发生了自发的菌落形成。RhG-CSF和rhGM-CSF刺激8例AML患者白血病集落形成。csf在两种培养中均具有加性效应。脑脊液均未诱导氧生成或吞噬活性。从这些结果，我们得出结论，这两种csf刺激白血病细胞的增殖而不诱导分化。
The effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) and recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) on primary human leukemic cells were studied. Phagocyte-depleted mononuclear cells containing more than 88% blasts were obtained from peripheral blood of 11 AML and 2 ALL patients and from bone marrow aspirates from 2 ALL patients. The leukemic cells were incubated with these CSF in suspension cultures or in methylcellulose cultures. In suspension cultures, the spontaneous proliferation was observed in 1 M4 patient. RhG-CSF stimulated the leukemic cell proliferation in 5 AML, cases and rhGM-CSF that in 4 AML cases. In methylcellulose cultures, spontaneous colony formation occurred in 3 M4 patients. RhG-CSF and rhGM-CSF stimulated the leukemic colony formation in 8 AML cases. The CSFs had an additive effect in both cultures. Neither CSF induced O2- production or phagocytic activity. From these results, we concluded that both CSFs stimulated the proliferation of leukemic cells without inducing differentiation.