RESTRICTED DIVERSITY OF V-GAMMA-9-JP REARRANGEMENTS IN UNSTIMULATED HUMAN GAMMA/DELTA T LYMPHOCYTE-T
RESTRICTED DIVERSITY OF V-GAMMA-9-JP REARRANGEMENTS IN UNSTIMULATED HUMAN GAMMA/DELTA T LYMPHOCYTE-T
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DOI：
10.1002/eji.1830220937
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发表时间：
1992-09-01
影响因子：
5.4
通讯作者：
GRANDCHAMP, B
中科院分区：
文献类型：
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作者：
DELFAU, MH;HANCE, AJ;GRANDCHAMP, B
已知人外周血γ/δ-T细胞的多样性受到优先使用编码V（γ）9（通常与JP相关）和V（δ）2的V基因的限制。我们发现，这些细胞的多样性进一步限制在交界区。首先，在含有V（γ）9-JP重排的所有γ/δ-T细胞的10%-30%中发现相同的重排。第二，尽管如此，绝大多数与该主要序列不同的V（γ）9-JP重排具有相同的长度或编码可变区，其长度仅相差一个氨基酸（+/-1）。总的来说，30%-50%的V（γ）9-JP重排具有编码具有氨基酸序列E VX EL的肽的接合区，其中EV主要但不排他地由种系V（γ）9序列编码，EL由JP编码。X氨基酸是可变的，但谷氨酰胺是过度代表的。V（γ）9-JP库的多样性在不同个体和不同年龄中是相当恒定的，包括外周血γ/δ-T细胞的出生后扩增之前、期间和之后。
The diversity of human peripheral blood gamma/delta-T cells is known to be limited by the preferential use of V genes coding for V(gamma)9 (usually linked to JP) and V(delta)2. We show that the diversity of these cells is further limited at the junctional region. First, an identical rearrangement is found in 10%-30% of all gamma/delta-T cells which contain V(gamma)9-JP rearrangements. Second, the vast majority of V(gamma)9-JP rearrangements which are different from this predominant sequence have, nevertheless, the same length or code for variable regions whose length differs by only one amino acid (+/- 1). Overall, 30%-50% of V(gamma)9-JP rearrangements have a junctional region which encodes for a peptide with the amino acid sequence E VX EL, in which EV is predominantly, but not exclusively, encoded by the germ-line V(gamma)9 sequence and EL is encoded by JP. The X amino acid is variable, but a glutamine is over-represented. The diversity of the V(gamma)9-JP repertoire is fairly constant in different individuals and at different ages, including before, during and after the post-natal expansion of peripheral blood gamma/delta-T cells.