IMMUNOLOGICAL AND NON-IMMUNOLOGIC GENERATION OF SUPEROXIDE FROM MAST-CELLS AND BASOPHILS
IMMUNOLOGICAL AND NON-IMMUNOLOGIC GENERATION OF SUPEROXIDE FROM MAST-CELLS AND BASOPHILS
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DOI：
10.1172/jci108917
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发表时间：
1978-01-01
影响因子：
15.9
通讯作者：
KALINER, M
中科院分区：
文献类型：
--
作者：
HENDERSON, WR;KALINER, M
Mediator release from rat peritoneal and human lung mast cells as well as human leukemic basophils was examined to determine whether super-oxide (O−2) was concomitantly generated. Immunologic or nonimmunologic stimulation of each preparation induced parallel release of histamine and O−2within 2 min. O−2production was quantitated by superoxide dismutase (SOD)-inhibitable chemiluminescence and cytochromecreduction. SOD was detected in basophil and mast cell lysates and was also released by rat mast cells stimulated by anti-IgE. Secretory granules isolated from purified rat mast cells released histamine, O−2, and SOD upon exposure to cations. Thus, both superoxide radicals and SOD may play a role in host defenses involved in immediate hypersensitivity reactions.Images
