Transcriptome Profiling and Molecular Pathway Analysis of Genes in Association with Salinity Adaptation in Nile Tilapia Oreochromis niloticus.
Transcriptome Profiling and Molecular Pathway Analysis of Genes in Association with Salinity Adaptation in Nile Tilapia Oreochromis niloticus.
复制标题
基因的转录组分析和分子途径分析与尼罗尼罗尼达尼罗尼罗尼氏菌的盐度适应性相关。
DOI：
10.1371/journal.pone.0136506
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Li E
中科院分区：
文献类型：
--
作者：
Xu Z;Gan L;Li T;Xu C;Chen K;Wang X;Qin JG;Chen L;Li E
尼罗罗非鱼Oreochromis niloticus是一种淡水鱼，但可以耐受各种盐度。利用RNA-Seq技术从分子水平上研究了鱼类在0、8和16（实际盐度单位，psu）盐度下的适应机制。根据基因表达的变化，将淡水到咸水的差异基因组合分为三类。在恒定变化类别（1）中，类固醇生物合成、类固醇激素生物合成、脂肪消化和吸收、补体和凝血级联反应受到盐度的显著影响，表明类固醇相关途径在响应盐度胁迫中的关键作用。在先变化后稳定的类别（2）中，核糖体、氧化磷酸化、过氧化物酶体增殖物激活受体的信号通路以及脂肪的消化吸收都随着盐度的增加而发生显著变化，表现出对环境中盐度变化的敏感性和对盐度变化的响应阈值。在稳定-变化类别（3）中，蛋白质输出、内质网蛋白质加工、紧密连接、甲状腺激素合成、抗原加工和呈递、糖酵解/糖异生和糖胺聚糖生物合成-硫酸角质素是发生显著变化的途径，表明这些途径对盐度变化的敏感性较低。本研究揭示了O. niloticus，并提供了一般指导，以了解盐驯化O。尼罗河。
Nile tilapia Oreochromis niloticus is a freshwater fish but can tolerate a wide range of salinities. The mechanism of salinity adaptation at the molecular level was studied using RNA-Seq to explore the molecular pathways in fish exposed to 0, 8, or 16 (practical salinity unit, psu). Based on the change of gene expressions, the differential genes unions from freshwater to saline water were classified into three categories. In the constant change category (1), steroid biosynthesis, steroid hormone biosynthesis, fat digestion and absorption, complement and coagulation cascades were significantly affected by salinity indicating the pivotal roles of sterol-related pathways in response to salinity stress. In the change-then-stable category (2), ribosomes, oxidative phosphorylation, signaling pathways for peroxisome proliferator activated receptors, and fat digestion and absorption changed significantly with increasing salinity, showing sensitivity to salinity variation in the environment and a responding threshold to salinity change. In the stable-then-change category (3), protein export, protein processing in endoplasmic reticulum, tight junction, thyroid hormone synthesis, antigen processing and presentation, glycolysis/gluconeogenesis and glycosaminoglycan biosynthesis—keratan sulfate were the significantly changed pathways, suggesting that these pathways were less sensitive to salinity variation. This study reveals fundamental mechanism of the molecular response to salinity adaptation in O. niloticus, and provides a general guidance to understand saline acclimation in O. niloticus.