Cross-talk between ciliary epithelium and trabecular meshwork cells in-vitro: a new insight into glaucoma.
Cross-talk between ciliary epithelium and trabecular meshwork cells in-vitro: a new insight into glaucoma.
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DOI：
10.1371/journal.pone.0112259
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Beit-Yannai E
中科院分区：
文献类型：
--
作者：
Lerner N;Beit-Yannai E
据推测，非色素纤毛上皮通过其神经内分泌活动调节眼压。为了验证这一假设，我们研究了与人非色素纤毛上皮细胞系（ODM-2）共培养对人小梁网（TM）细胞系（NTM）的影响。在共培养系统中研究ODM-2和NTM细胞间的细胞串扰，共培养5 ~ 60min，共培养2、4、8h，然后取出共培养分析。分析了ERK和p38丝裂原活化蛋白激酶（MAPK）通路以及TM磷酸酶和基质金属蛋白（MMPs）的活性。酸性和碱性磷酸酶活性测定采用DiFMUP（6,8 -二氟-4-甲基伞状叶磷酸）测定法。明胶酶谱法测定MMP水平。NTM细胞暴露于ODM-2细胞后，在共培养5min内，NTM细胞MAPK信号转导通路被激活。ERK1/ERK2和p38的磷酸化在10和15min时达到峰值，然后随着时间的推移而下降。共培养4h后，ODM-2与NTM细胞相互作用促进了NTM细胞中MMP-9的表达。我们的发现为ODM-2和NTM细胞之间确实发生串扰的假设提供了支持。未来的研究应旨在确定MMP系统，MAPK激酶和磷酸酶之间的关系。操纵这些信号分子和相关的NTM信号转导途径可能为开发改进的青光眼治疗方法提供靶点。
It is assumed that the non-pigmented ciliary epithelium plays a role in regulating intraocular pressure via its neuroendocrine activities. To test this hypothesis, we investigated the effect on a human trabecular meshwork (TM) cell line (NTM) of co-culture with a human non-pigmented ciliary epithelium cell line (ODM-2). The cellular cross-talk between ODM-2 and NTM cells was studied in a co-culture system in which the two cell types were co-cultured for 5 to 60min or 2, 4 and 8h and then removed from the co-culture and analyzed. Analyses of the ERK and p38 mitogen-activated protein kinase (MAPK) pathways and of the activity of TM phosphatases and matrix metalloproteins (MMPs) were performed. Acid and alkaline phosphatase activity was determined by the DiFMUP (6, 8-difluoro-4-methylumbelliferyl phosphate) assay. MMP levels were determined by gelatin zymography. Exposure of NTM cells to ODM-2 cells led to the activation of the MAPK signal transduction pathways in NTM cells within 5min of co-culture. Phosphorylation of ERK1/ERK2 and p38 peaked at 10 and 15min and then decreased over time. Interaction between ODM-2 and NTM cells promoted the expression of MMP-9 in the NTM cells after 4h of co-culture. Our findings provide support for the hypothesis that crosstalk does indeed take place between ODM-2 and NTM cells. Future studies should be designed to determine the relationship between the MMP system, MAPK kinases and phosphatases. Manipulation of these signaling molecules and the related NTM signal transduction pathways may provide targets for developing improved treatments for glaucoma.
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DOI：
10.1073/pnas.83.22.8754
发表时间：
1986-11-01
影响因子：
11.1
作者：
COCAPRADOS, M;WAX, MB
通讯作者：
WAX, MB
DOI：
10.4049/jimmunol.0803357
发表时间：
2009-02-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kumar M;Makonchuk DY;Li H;Mittal A;Kumar A
通讯作者：
Kumar A
DOI：
10.1016/j.biocel.2003.12.007
发表时间：
2004-05-01
影响因子：
4
作者：
Marchenko, ND;Marchenko, GN;Strongin, AY
通讯作者：
Strongin, AY
影响因子：
5.1
作者：
Loeser RF;Erickson EA;Long DL
通讯作者：
Long DL
影响因子：
4.4
作者：
Hosseini, M;Rose, AY;Acott, TS
通讯作者：
Acott, TS