MUSCLE LIM PROTEIN, A NOVEL ESSENTIAL REGULATOR OF MYOGENESIS, PROMOTES MYOGENIC DIFFERENTIATION
MUSCLE LIM PROTEIN, A NOVEL ESSENTIAL REGULATOR OF MYOGENESIS, PROMOTES MYOGENIC DIFFERENTIATION
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DOI：
10.1016/0092-8674(94)90192-9
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发表时间：
1994-10-21
期刊：
影响因子：
64.5
通讯作者：
CARONI, P
中科院分区：
文献类型：
--
作者：
ARBER, S;HALDER, G;CARONI, P
肌肉LIM蛋白（Muscle LIM protein，MLP）是一种新的肌生成正调控因子。其表达和果蝇同源物DMLP 1的表达在横纹肌中富集，并与肌源性分化相一致。在没有MLP的情况下，诱导的C2细胞表达肌细胞生成素，但不能退出细胞周期和分化。MLP在C2成肌细胞中的过表达增强肌源性分化并降低其对TGF β的敏感性。与MLP一样，MLP和非肌肉LIM蛋白的单一LIM结构域缺失突变体促进肌源性分化。在3 T3成纤维细胞中，相同的LIM蛋白阻止佛波酯诱导的DNA复制抑制。这些结果确立了MLP作为肌生成的重要启动子，并表明LIM-唯一的蛋白质通过类似的机制来调节细胞分化的各个方面。
Muscle LIM protein (MLP) is a novel positive regulator of myogenesis. Its expression and that of its Drosophila homolog DMLP1 are enriched in striated muscle and coincide with myogenic differentiation. in the absence of MLP, induced C2 cells express myogenin but fail to exit from the cell cycle and to differentiate. Overexpression of MLP in C2 myoblasts potentiates myogenic differentiation and reduces its sensitivity to TGF beta. Like MLP, single LIM domain deletion mutants of MLP and nonmuscle LIM-only proteins promote myogenic differentiation. In 3T3 fibroblasts, the same LIM proteins prevent phorbol ester-induced inhibition of DNA replication. These results establish MLP as an essential promoter of myogenesis and suggest that LIM-only proteins act via similar mechanisms to regulate aspects of cell differentiation.