Production of lignocellulolytic enzymes and enhancement of in vitro digestibility during solid state fermentation of wheat straw by Phlebia floridensis
Production of lignocellulolytic enzymes and enhancement of in vitro digestibility during solid state fermentation of wheat straw by Phlebia floridensis
复制标题
DOI：
10.1016/j.biortech.2010.07.042
复制
发表时间：
2010-12-01
影响因子：
11.4
通讯作者：
Arora, Daljit Singh
中科院分区：
文献类型：
--
作者：
Sharma, Rakesh Kumar;Arora, Daljit Singh
利用白色腐真菌降解小麦秸秆有可能提高其消化率，从而提高其作为动物饲料的价值。为优化黄花脉鞭菌固态发酵小麦秸秆产木质纤维素降解酶的条件，提高其体外消化率，设计了响应面法（RSM）试验。研究了水分、无机氮源（NH 4Cl）和麦芽提取物对小麦秸秆木质纤维素分解酶、化学成分变化及消化率的影响。随着水分含量的增加，漆酶的产量增加了34倍，而锰过氧化物酶的最佳产生在几乎等量（50-55毫克/克WS）的氯化铵和麦芽提取物的存在下。这些补充物还显著（p < 0.05）提高了CMCase和木聚糖酶的产生。体外消化率提高了近50%，木质素和总有机物分别损失27.6%和14.6%。本研究结果表明，植物纤维素酶是一种高效的微生物产生木质纤维素降解酶，同时提高体外消化率的小麦秸秆。(C)2010爱思唯尔有限公司版权所有。
Degradation by white rot fungi has the potential to increase digestibility of wheat straw and thus improve its value as animal feed. To optimize conditions for production of lignocellulolytic enzymes by Phlebia floridensis during solid state fermentation of wheat straw along with enhancement of in vitro digestibility, a response surface methodology (RSM) based experiment was designed. Effect of moisture content, inorganic nitrogen source (NH4Cl) and malt extract on lignocellulolytic enzymes, changes in chemical constituents and digestibility of wheat straw was evaluated. With increase in moisture content, laccase production increased up to 34-fold, while Manganese peroxidase was optimally produced in the presence of almost equal amount (50-55 mg/g of WS) of NH4Cl and malt extract. These supplements also significantly (p < 0.05) enhanced the production of CMCase and xylanase. In vitro digestibility was increased by almost 50% with a loss of 27.6% and 14.6% in lignin and total organic matter, respectively. The present findings revealed P. floridensis to be an efficient organism for lignocellulolytic enzymes production and simultaneous enhancement in in vitro digestibility of wheat straw. (C) 2010 Elsevier Ltd. All rights reserved.