Transcriptomic changes in western mosquitofish (Gambusia affinis) liver following benzo[a]pyrene exposure
Transcriptomic changes in western mosquitofish (Gambusia affinis) liver following benzo[a]pyrene exposure
复制标题
苯并[a]芘暴露后西方蚊鱼（Gambusia affinis）肝脏的转录组变化
DOI：
10.1007/s11356-020-08571-0
复制
发表时间：
2020-04-13
影响因子：
5.8
通讯作者：
Xie，Shaolin
中科院分区：
文献类型：
--
作者：
Feng，Yongyong;Zhou，Aiguo;Xie，Shaolin
广泛分布的西方蚊虫(Gambusia Affinis)已被用作多环芳烃(PAHs)等环境应激源危害评估的新模式物种。然而，大多数多环芳烃的研究使用。亲和力依赖于有针对性的基于生物标记物的分析，因此可能无法充分解决应激源毒性机制的复杂性。在本研究中，对G。在多环芳烃模型苯并[a]芘(BaP)(100μg/L)中暴露20天后，用HiSeq XTen测序仪进行亲和试验。在对照和暴露于BaP的文库中，分别获得了58,156,233和51,825,467个干净的核苷酸读数，平均N50长度为1419个碱基。此外，后半段。亲缘关系暴露20天后，169个基因在肝脏中上调，176个基因在肝脏中下调。对所有基因进行基因本体论(GO)和京都基因与基因组百科全书(KEGG)途径分析，以确定基因的生物学功能和过程。结果表明，差异表达基因主要与免疫途径和代谢相关途径有关。有趣的是，几乎所有与免疫相关的途径都上调了，而代谢途径却下调了。最后，采用实时定量聚合酶链式反应(qRT-PCR)检测DGE分析证实的12个基因的表达水平。这些结果表明，BaP可损害机体免疫功能，增加对所有可用储能的消耗，从而激活解毒机制。阿菲尼斯。到目前为止，本研究是首次对肝脏OFG进行全转录组测序分析。有报道称，亲和力接触到了苯并苯。
Widely distributed western mosquitofish (Gambusia affinis) has been used as a new model species for hazard assessment of environmental stressors such as polycyclic aromatic hydrocarbons (PAHs). However, most of the PAH studies usingG. affinisrely on targeted biomarker-based analysis, and thus may not adequately address the complexity of the toxic mechanisms of the stressors. In the present study, the whole transcriptional sequencing ofG. affinisliver after exposure to a PAH model, benzo[a]pyrene (BaP) (100 μg/L), for 20 days was performed by using the HiSeq XTen sequencers. In total, 58,156,233 and 51,825,467 clean nucleotide reads were obtained in the control and BaP-exposed libraries, respectively, with average N50 lengths of 1419 bp. In addition, afterG. affiniswas exposed for 20 days, 169 genes were upregulated, and 176 genes were downregulated in liver. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were applied to all the genes to determine the genes’ biological functions and processes. The results clearly showed that the differentially expressed genes were mainly related to immune pathways and metabolic correlation pathways. Interestingly, almost all the pathways related with the immunity were upregulated, while the metabolism pathways were downregulated. Lastly, quantitative real-time PCR (qRT-PCR) was performed to measure expressional levels of twelve genes confirmed through the DGE analysis. These results demonstrate that BaP damages immunity and enhances the consumption of all available energy storage to activate mechanisms of the detoxification inG. affinis. Up until now, the present study is the first time that a whole transcriptome sequencing analysis in the liver ofG. affinisexposed to BaP has been reported.