A comparison of the fluorescence of the Y base of yeast tRNA-Phe in solution and in crystals.
A comparison of the fluorescence of the Y base of yeast tRNA-Phe in solution and in crystals.
复制标题
溶液和晶体中酵母 tRNA-Phe Y 碱基荧光的比较。
DOI：
10.1021/bi00682a040
复制
发表时间：
1975
期刊：
影响因子：
2.9
通讯作者：
C. Cantor
中科院分区：
文献类型：
--
作者：
R. Langlois;S. Kim;C. Cantor
已知酵母tRNA-Phe的Y碱基的荧光性质对环境相当敏感。酵母tRNA-Phe中Y碱基的荧光寿命在斜方晶体和这些晶体生长的母液中是相同的。它比在稀释的tRNA溶液中观察到的寿命高10%。该微小变化是结晶介质中异丙醇引起的溶剂效应。异丙醇不改变Y碱的发色团的可及性，如通过溶液中的碘化物淬灭速率所测量的。完整的tRNA-Phe中的可及性比核糖核酸酶消化物中的可及性小得多。因此，在该方法的灵敏度的限度内，Y发色团在溶液中和晶体中占据相同的环境，并且它必须至少部分地被掩埋。
The fluorescence properties of the Y base of yeast tRNA-Phe are known to be quite sensitive to the environment. The fluorescence lifetime of the Y base in yeast tRNA-Phe is identical in orthorhombic crystals and in the mother liquor from which these crystals are grown. It is 10% higher than the lifetime observed in dilute solutions of tRNA. This small change is a solvent effect due to isopropyl alcohol in the crystallization medium. Isopropyl alcohol does not change the accessibility of the chromophore of the Y base as measured by iodide quenching rates in solution. The accessibility in intact tRNA-Phe is much less than in a ribonuclease digest. Thus, within the limits of the sensitivity of the method, the Y chromophore occupies the same environment in solution and in the crystal and it must be at least partially buried.