Analysis of tobamovirus multiplication in Arabidopsis thaliana mutants defective in TOM2A homologues
Analysis of tobamovirus multiplication in Arabidopsis thaliana mutants defective in TOM2A homologues
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DOI：
10.1099/vir.0.2008/000539-0
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发表时间：
2008-06-01
影响因子：
3.8
通讯作者：
Ishikawa, Masayuki
中科院分区：
文献类型：
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作者：
Fujisaki, Koki;Kobayashi, Soko;Ishikawa, Masayuki
拟南芥的 TOM2A 基因编码四次跨膜蛋白，该蛋白是烟草花病毒 TMV-Cg 有效增殖所必需的。在本研究中，研究了拟南芥中烟草花叶病毒增殖中三个 TOM2A 同源物的参与情况。三个同源物中的 T-DNA 插入突变，无论是单独还是组合，都不会影响 TMV-Cg 的增殖，而在 tom2a 遗传背景下，某些组合会降低 TMV-Cg 的增殖。该结果表明TOM2A同源物在增强TMV-Cg增殖方面具有功能，但其贡献远小于TOM2A。有趣的是，据我们检查，另一种烟草花叶病毒（番茄花叶病毒）的增殖并未受到 TOM2A 及其同源物突变组合的严重影响。
The TOM2A gene of Arabidopsis thaliana encodes a four-pass transmembrane protein that is required for efficient multiplication of a tobamovirus, TMV-Cg. In this study, the involvement of three TOM2A homologues in tobamovirus multiplication in A. thaliana was examined. T-DNA insertion mutations in the three homologues, separately or in combination, did not affect TMV-Cg multiplication, whereas, in the tom2a genetic background, some combinations reduced it. This result suggests that the TOM2A homologues are functional in enhancing TMV-Cg multiplication, but their contribution is much less than TOM2A. Interestingly, the multiplication of another tobamovirus, Tomato mosaic virus, was not drastically affected by any combinations of the mutations in TOM2A and its homologues as far as we examined.