Prevalence, Virulence Genes, Antimicrobial Susceptibility, and Genetic Diversity of Bacillus cereus Isolated From Pasteurized Milk in China.
Prevalence, Virulence Genes, Antimicrobial Susceptibility, and Genetic Diversity of Bacillus cereus Isolated From Pasteurized Milk in China.
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中国巴氏杀菌乳中蜡状芽孢杆菌的流行情况、毒力基因、耐药性及遗传多样性
DOI：
10.3389/fmicb.2018.00533
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发表时间：
2018
影响因子：
5.2
通讯作者：
Wu H
中科院分区：
文献类型：
--
作者：
Gao T;Ding Y;Wu Q;Wang J;Zhang J;Yu S;Yu P;Liu C;Kong L;Feng Z;Chen M;Wu S;Zeng H;Wu H
蜡样芽孢杆菌是一种常见和重要的食源性病原体，可在各种食品中发现。由于低温灭菌时间短，巴氏灭菌不足以完全消除B。牛奶中的蜡状芽孢杆菌，从而造成严重的经济损失和食品安全问题。因此，对B进行风险评估至关重要。巴氏消毒牛奶中的蜡状芽孢杆菌。在本研究中，我们分离了B。对我国不同地区巴氏杀菌乳样品中蜡样芽胞杆菌的污染状况、毒力基因的存在、耐药性及B的遗传多态性进行了分析。蜡状菌分离物。有趣的是，70个样本（27%）被发现被B污染。蜡状芽孢杆菌的平均污染水平为111 MPN/g。针对10个肠促炎基因（hblA、hblC、hblD、nheA、nheB、nheC、cytK、entFM、bceT和hly II）和1个催吐基因（cesB）评估毒力基因的分布。45%的菌株携带hblACD基因，93%的菌株携带nheABC基因簇。cytK、entFM、bceT、hlyII和cesB基因的阳性率分别为73%、96%、75%、54%和5%。药敏结果显示，大部分菌株对β-内酰胺类抗生素和利福平耐药，而对环丙沙星、庆大霉素和氯霉素敏感。总的多重耐药人群约占34%。此外，B.巴氏杀菌乳中的蜡样芽胞杆菌分离株显示出较高的遗传多样性。本研究结果为B的流行、污染水平及特征提供了第一个参考依据。从我国巴氏杀菌乳中分离到一株蜡状芽孢杆菌，提示存在B的潜在高风险。蜡状芽孢杆菌对公共卫生和乳品工业的影响。
Bacillus cereus is a common and important food-borne pathogen that can be found in various food products. Due to low-temperature sterilization for a short period of time, pasteurization is not sufficient for complete elimination of B. cereus in milk, thereby cause severe economic loss and food safety problems. It is therefore of paramount importance to perform risk assessment of B. cereus in pasteurized milk. In this study, we isolated B. cereus from pasteurized milk samples in different regions of China, and evaluated the contamination situation, existence of virulence genes, antibiotic resistance profile and genetic polymorphism of B. cereus isolates. Intriguingly, 70 samples (27%) were found to be contaminated by B. cereus and the average contamination level was 111 MPN/g. The distribution of virulence genes was assessed toward 10 enterotoxigenic genes (hblA, hblC, hblD, nheA, nheB, nheC, cytK, entFM, bceT, and hlyII) and one emetic gene (cesB). Forty five percent strains harbored enterotoxigenic genes hblACD and 93% isolates contained nheABC gene cluster. The positive rate of cytK, entFM, bceT, hlyII, and cesB genes were 73, 96, 75, 54, and 5%, respectively. Antibiotic susceptibility assessment showed that most of the isolates were resistant to β-lactam antibiotics and rifampicin, but susceptible to other antibiotics such as ciprofloxacin, gentamicin and chloramphenicol. Total multidrug-resistant population was about 34%. In addition, B. cereus isolates in pasteurized milk showed a high genetic diversity. In conclusion, our findings provide the first reference on the prevalence, contamination level and characteristics of B. cereus isolated from pasteurized milk in China, suggesting a potential high risk of B. cereus to public health and dairy industry.
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影响因子：
5.2
作者：
Ehling-Schulz M;Frenzel E;Gohar M
通讯作者：
Gohar M
影响因子：
2.1
作者：
Ehling-Schulz, Monika;Guinebretiere, Marie-Helene;Svensson, Birgitta
通讯作者：
Svensson, Birgitta
影响因子：
4.4
作者：
Asano, S;Nukumizu, Y;Yamamoto, T
通讯作者：
Yamamoto, T
影响因子：
5.3
作者：
Bartoszewicz, Marek;Marjanska, Paulina Sylwia
通讯作者：
Marjanska, Paulina Sylwia
影响因子：
2.8
作者：
Bogdanova, ES;Bass, IA;Nikiforov, VG
通讯作者：
Nikiforov, VG