Microsatellites mapping for non-model species with chromosomal rearrangement: a case study in the frog Quasipaa boulengeri (Anura: Dicroglossidae).
Microsatellites mapping for non-model species with chromosomal rearrangement: a case study in the frog Quasipaa boulengeri (Anura: Dicroglossidae).
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DOI：
10.1139/gen-2016-0200
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发表时间：
2017-07
期刊：
影响因子：
3.1
通讯作者：
Xiuyun Yuan;Siqi Yuan;Ya Liu;Yun Xia;Xiaomao Zeng
中科院分区：
文献类型：
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作者：
Xiuyun Yuan;Siqi Yuan;Ya Liu;Yun Xia;Xiaomao Zeng
基因作图是了解基因进化和细胞遗传学的重要资源。具有已知遗传图谱或基因组序列的模式物种允许选择所需染色体上的遗传标记，而如果没有这样的参考，则很难在非模式物种的染色体上定位这些标记。一种蛙类，Quasipaa boulengeri，显示出染色体重排多态，使其成为抑制重组介导的染色体物种形成的一个迷人的模式。然而，在其重排的染色体上还没有找到任何标记。我们提出了一套完整的基于机械显微切割和染色体扩增技术的微卫星图谱绘制方案。按照这一方法，我们在染色体水平上定位了71个布伦格里栎微卫星。总共有8个基因座定位于重排染色体，另外63个基因座可能附着在其他染色体上。这些微卫星可以用来比较昆仑栎属的基因流和验证染色体抑制重组假说。这种整合的方法可以有效地用于非模式物种的基因定位。
Gene mapping is an important resource for understanding the evolution of genes and cytogenetics. Model species with a known genetic map or genome sequence allow for the selection of genetic markers on a desired chromosome, while it is hard to locate these markers on chromosomes of non-model species without such references. A frog species, Quasipaa boulengeri, shows chromosomal rearrangement polymorphisms, making itself a fascinating model for chromosomal speciation mediated by suppressed recombination. However, no markers have been located on its rearranged chromosomes. We present a complete protocol to map microsatellites based on mechanical microdissection and chromosome amplification techniques. Following this protocol, we mapped 71 microsatellites of Q. boulengeri at the chromosome level. In total, eight loci were assigned to rearranged chromosomes, and the other 63 loci might attach to other chromosomes. These microsatellites could be used to compare the gene flow and verify the chromosomal suppressed recombination hypothesis in Q. boulengeri. This integrated protocol could be effectively used to map genes to chromosomes for non-model species.