Hypertrehalosemic hormone in a cockroach: molecular cloning and expression.
Hypertrehalosemic hormone in a cockroach: molecular cloning and expression.
复制标题
蟑螂的高海藻糖激素：分子克隆和表达。
DOI：
10.1016/s0303-7207(97)00078-6
复制
发表时间：
1997
影响因子：
4.1
通讯作者：
Bradfield,JY
中科院分区：
文献类型：
--
作者：
Lewis,DK;Jezierski,MK;Keeley,LL;Bradfield,JY
高海藻糖血症激素（Hypertrehalosemic hormone，HTH）是一种神经肽，属于促脂激素/红色素浓集激素（adipokinetic hormone/red pigment-concentrating hormone，AKH/RPCH）家族，能刺激海藻糖的合成，而海藻糖是许多昆虫的主要血糖。从HTH的内分泌来源心脏体（CC）中克隆了蟑螂Blaberus discoidalis的preproHTH，并将preproHTH的推导序列和组织与其他AKH/RPCH前体进行比较。如DNA序列分析所预测的，PreproHTH mRNA的长度被确定为约0.5kb。北方印迹分析CC、腹神经索、脑和脂肪体仅在CC中检测到HTH-mRNA。根据年龄、性别和交配确定CC中HTH转录物的水平。HTH信息是最丰富的CC在头几天的成年生活在男女，然后下降了50%，并保持稳定。CC中的HTH-mRNA水平对交配没有反应。
Hypertrehalosemic hormone (HTH) is a neuropeptide in the adipokinetic hormone/red pigment-concentrating hormone (AKH/RPCH) family that stimulates the synthesis of trehalose, the main blood sugar of many insects. The preproHTH of the cockroach Blaberus discoidalis was cloned from the corpora cardiaca (CC), the endocrine source for HTH, and the deduced sequence and organization of preproHTH were compared with other AKH/RPCH precursors. PreproHTH mRNA was determined to be ∼0.5 kb in length as predicted by DNA sequence analysis. Northern blot analysis of the CC, ventral nerve cord, brain and fat body detected HTH-mRNA only in the CC. Levels of the HTH transcript in the CC were determined according to age, gender and mating. The HTH message was most abundant in the CC during the first several days of adult life in both sexes, then declined by 50% and were stable. HTH-mRNA levels in the CC did not respond to mating.