Germination properties as marker events characterizing later stages of Bacillus subtilis spore formation
Germination properties as marker events characterizing later stages of Bacillus subtilis spore formation
复制标题
发芽特性作为表征枯草芽孢杆菌孢子形成后期的标记事件
DOI：
10.1128/jb.141.2.786-792.1980
复制
发表时间：
1980
影响因子：
3.2
通讯作者：
J. Mandelstam
中科院分区：
文献类型：
--
作者：
P. Dion;J. Mandelstam
在孢子形成过程中的各个阶段，通过冷处理或甲苯对孢子囊进行冲击，并检查前孢子的萌发要求。诱导孢子形成后 5 小时 (t5) 自发萌发；也就是说，它在没有添加任何起始物的情况下发生。 t5之后，在阶段V和VI期间，自发萌发的能力逐渐减弱，并且孢子需要外部添加的萌发剂。在 t6 时，这种需要可以通过 L-丙氨酸或 KCl、葡萄糖和果糖的混合物来满足。到了t8，后者的反应已经消失了。孢子只有L-丙氨酸才能萌发，而且反应要慢得多。氯霉素实验表明，t6 和 t8 之间出现的孢子萌发特性是 t5 之前发生的蛋白质合成事件的表达。尽管发芽要求与耐热性几乎同时发展，但它们可能与野生型和突变细胞的耐热性无关。发育中孢子的萌发特性是表征孢子形成后期的附加标记事件，如下： (i) 自发萌发（直至第四阶段结束）； (ii) KCl-葡萄糖-果糖或L-丙氨酸满足的发芽要求（阶段V）； (iii) 仅用 L-丙氨酸减缓发芽反应（VI 阶段）。
At various stages during spore formation sporangia were shocked by cold treatment or with toluene, and the germination requirements of the prespores were examined. Up to 5 h after induction of sporulation (t5) germination was spontaneous; i.e., it occurred without any added germinants. After t5, during stages V and VI, the capacity for spontaneous germination diminished progressively, and the spores acquired a need for externally added germinants. At t6 this need was satisfied by either L-alanine or a mixture of KCl, glucose, and fructose. By t8, the latter response had disappeared. The spores germinated only with L-alanine, and the response was much slower. Experiments with chloramphenicol showed that the germination properties of the spores appearing between t6 and t8 were the expression of events in protein synthesis that had occurred before t5. Although the germination requirements developed at about the same time as heat resistance, they could be dissociated from heat resistance in wild-type and mutant cells. The germination properties of the developing spores are additional marker events characterizing the later stages of sporulation, as follows: (i) spontaneous germination (up to the end of stage IV); (ii) germination requirements that are satisfied by KCl-glucose-fructose or L-alanine (stage V); and (iii) slow germination response with L-alanine only (stage VI).