Dietary protein and lactose increase translation initiation factor activation and tissue protein synthesis in neonatal pigs
Dietary protein and lactose increase translation initiation factor activation and tissue protein synthesis in neonatal pigs
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DOI：
10.1152/ajpendo.00351.2005
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发表时间：
2006-02-01
影响因子：
5.1
通讯作者：
Davis, TA
中科院分区：
文献类型：
--
作者：
Frank, JW;Escobar, J;Davis, TA
注射氨基酸和胰岛素的猪肌肉和肝脏的蛋白质合成和真核细胞起始因子(EIF)活性增加。为研究肠道蛋白质和碳水化合物对蛋白质合成的影响，对42头体重为1.7 kg的仔猪饲喂含5、15和25g蛋白质的等热量奶日粮。Kg Body wt(-1)(.)天(-1))和两个水平的乳糖(低=11和高=23克(.)Kg Body wt(-1)(.)天(-1))，从1日龄到6日龄。第7天，断食4h后灌胃，测定组织蛋白合成率和mRNA翻译的生物标志物。仔猪生长和肌肉、肝脏蛋白质合成率随日粮蛋白质含量的增加而增加，在15g(kg·d)时趋于平稳。Kg Body wt(-1)(.)天(-1)(P<；0.001)。高乳糖饲喂组猪的生长速度更快(P=0.07)。血浆胰岛素水平最低的是饲喂5g的猪。Kg Body wt(-1)(.)-1天蛋白质(P<；0.0001)。血浆支链氨基酸随着蛋白质摄入量的增加而增加(P<；0.0001)。肌肉(P<；0.001)和肝脏(P<；
Protein synthesis and eukaryotic initiation factor ( eIF) activation are increased in muscle and liver of pigs parenterally infused with amino acids and insulin. To examine the effects of enteral protein and carbohydrate on protein synthesis, pigs ( n = 42, 1.7 kg body wt) were fed isocaloric milk diets containing three levels of protein ( 5, 15, and 25 g (.) kg body wt(-1) (.) day(-1)) and two levels of lactose ( low = 11 and high = 23 g (.) kg body wt(-1) (.) day(-1)) from 1 to 6 days of age. On day 7, pigs were gavage fed after 4-h food deprivation, and tissue protein synthesis rates and biomarkers of mRNA translation were assessed. Piglet growth and protein synthesis rates in muscle and liver increased with dietary protein and plateaued at 15 g (.) kg body wt(-1) (.) day(-1) ( P < 0.001). Growth tended to be greater in high-lactose-fed pigs ( P = 0.07). Plasma insulin was lowest in pigs fed 5 g (.) kg body wt(-1) (.) day(-1) protein ( P < 0.0001). Plasma branched-chain amino acids increased as protein intake increased ( P < 0.0001). Muscle ( P < 0.001) and liver ( P