Microsatellite markers for population genetic studies in Aedes aegypti (Diptera: Culicidae) from Cote d'Ivoire:: evidence for a microgeographic genetic differentiation of mosquitoes from Bouake
Microsatellite markers for population genetic studies in Aedes aegypti (Diptera: Culicidae) from Cote d'Ivoire:: evidence for a microgeographic genetic differentiation of mosquitoes from Bouake
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DOI：
10.1016/s0001-706x(02)00028-1
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发表时间：
2002-04-01
期刊：
影响因子：
2.7
通讯作者：
Cuny, G
中科院分区：
文献类型：
--
作者：
Ravel, S;Hervé, JP;Cuny, G
在西非，埃及伊蚊（双翅目：库蚊科）（Linnaeus， C., 1762）。Zweyter Theil, enhalt Beschreibungen veschiedener, Naturalien。见：Hasselquist， F.（编），Reise nach Palastina In den Jahren von 1749 bis 1752，罗斯托克，德国，第267-606页)代表黄热病的主要媒介。这项研究报告了利用微卫星标记根据南北样带对来自科特迪瓦的各种埃及伊蚊种群进行表征，并对蚊子进行了时间遗传调查。三个微卫星位点用于分析来自四个不同地方的个体：卡波洛、布瓦凯和阿比让的两个不同地区。我们发现除了阿比让的两个种群外，这四个种群在基因上是不同的。在Bouake种群中，两个隐种的共存，在形态上无法区分，似乎解释了观察到的广泛的杂合子缺乏。相隔1年的布瓦克蚊子比较表明，随着时间的推移，该种群的结构发生了巨大变化。综上所述，这些结果表明微卫星标记可用于在微地理尺度上鉴定埃及伊蚊的不同种群，并可用于评估蚊子种群的时间变化。(C) 2002 Elsevier Science B.V.版权所有
In West Africa, Aedes aegypti (Diptera: Culicidae) (Linnaeus, C., 1762. Zweyter Theil, enhalt Beschreibungen veschiedener wichtiger Naturalien. In: Hasselquist, F. (Ed.), Reise nach Palastina in den Jahren von 1749 bis 1752, Rostock, Germany, pp. 267-606) represents the principal vector, of yellow fever. This study reports the use of microsatellite markers to characterise various A. aegypti populations from Cote d'Ivoire according to a north-south transect, and to perform a temporal genetic survey of the mosquitoes. Three microsatellite loci were used to analyse individuals from four different places: Kabolo, Bouake, and two different districts of Abidjan. We found that the four populations are genetically distinct except the two Abidjan populations. In the Bouake population, the coexistence of two cryptic species, not morphologically distinguishable, seems to account for the extensive heterozygote deficiency observed. Comparison of mosquitoes from Bouake I year apart indicated that a dramatic change occurred in the structuring of this population over time. Taken together these results indicate that microsatellite markers could be useful for identifying various populations of A. aegypti on a microgeographic scale and to assess for temporal variation within mosquito populations. (C) 2002 Elsevier Science B.V. All rights reserved.