Characterization of microsatellite loci isolated in Mountain Plover (Charadrius montanus)

Characterization of microsatellite loci isolated in Mountain Plover (Charadrius montanus)
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DOI:
10.1111/j.1471-8286.2007.01709.x
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发表时间:
2007-09-01
期刊:
MOLECULAR ECOLOGY NOTES
影响因子:
--
通讯作者:
Oyler-Mccance, S. J.
Oyler-Mccance, S. J.
中科院分区:
其他
文献类型:
--
作者:
John, J. St.;Kysela, R. F.;Oyler-Mccance, S. J.

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为山普洛弗开发了15个微卫星位点的引物,该物种的分布和丰度在过去30年中急剧减少。在筛选的126个个体中,从四个繁殖地点收集的物种范围内,多态性水平范围从每个基因座2到13个等位基因。没有两个位点被发现是连锁的,虽然一个位点揭示了显着偏离哈代-温伯格平衡。这些微卫星位点可用于群体遗传学研究,最终有助于管理工作的山普洛弗。此外,这些标记可以潜在地用于调查山普洛弗的交配系统的研究。
Primers for 15 microsatellite loci were developed for Mountain Plover, a species whose distribution and abundance have been reduced drastically in the past 30 years. In a screen of 126 individuals collected from four breeding locales across the species' range, levels of polymorphism ranged from two to 13 alleles per locus. No two loci were found to be linked, although one locus revealed significant departures from Hardy-Weinberg equilibrium. These microsatellite loci can be used in population genetic studies, ultimately aiding in management efforts for Mountain Plover. Additionally, these markers can potentially be used in studies investigating the mating system of Mountain Plover.