Deficit of galanin-like immunostaining in the median eminence of adult hypothyroid rats.
Deficit of galanin-like immunostaining in the median eminence of adult hypothyroid rats.
复制标题
成年甲状腺功能减退大鼠正中隆起的甘丙肽样免疫染色缺陷。
作者：
L. Giardino;Antonino Velardo;Andrea Gallinelli;L. Calzà
在本文中，我们描述了出生后 52 天甲状腺功能减退的大鼠下丘脑中甘丙肽 (GAL) 样免疫染色的修饰。手术切除甲状腺后第 21 天，正中隆起的 GAL 免疫反应纤维染色减少，第 84 天几乎完全消失。 GAL 免疫反应性分布在下丘脑的其他区域，例如下丘脑前部和背内侧核仅受到甲状腺激素缺乏的轻微影响，而对照组和甲状腺功能减退大鼠的延髓（迷走神经复合体）的 GAL 染色是相同的。在甲状腺功能减退秋水仙碱治疗的大鼠中，我们无法对室旁核（PVN）中的 GAL 免疫反应性神经元进行染色。催产素和加压素样物质存在于大细胞神经元中，甲状腺功能减退大鼠的染色模式与对照动物相同。我们的数据显示成年甲状腺功能减退大鼠的 PVN 和中位隆起的 GAL 样免疫反应性表达显着减少。讨论了这种缺陷在甲状腺功能减退大鼠中观察到的 GH 分泌障碍发病机制中可能的作用。
In this paper we describe the modification of the galanin (GAL)-like immunostaining in the hypothalamus of rats, which were made hypothyroid at 52 days after birth. On 21st day after the surgical ablation of the thyroid gland, the staining of the GAL-immunoreactive fibers in the median eminence decreased and on the 84th day disappeared almost totally. The GAL-immunoreactive distribution in other areas of the hypothalamus, e.g. the anterior hypothalamus and the dorsomedial nucleus, is only slightly affected by the absence of thyroid hormones, whereas the GAL-staining of medulla oblongata (vagal complex) is equal in both control and hypothyroid rats. In hypothyroid colchicine-treated rats, we were unable to stain GAL-immunoreactive neurons in the paraventricular nucleus (PVN). Oxytocin- and vasopressin-like material was present in the magnocellular neurons and the staining pattern in hypothyroid rats was the same as that of control animals. Our data show a marked reduction in the expression of the GAL-like immunoreactivity of the PVN and median eminence of adult hypothyroid rats. The possible role of this deficit in the pathogenesis of the GH secretion impairment that is observed in hypothyroid rats is discussed.