THE NUCLEOCAPSID OF BERNE VIRUS
THE NUCLEOCAPSID OF BERNE VIRUS
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DOI：
10.1099/0022-1317-66-6-1287
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发表时间：
1985-01-01
影响因子：
3.8
通讯作者：
WEISS, M
中科院分区：
文献类型：
--
作者：
HORZINEK, MC;EDERVEEN, J;WEISS, M
在伯尔尼病毒感染的细胞和梯度纯化的病毒体中检测到 MW 为 20,000 (20K) 和 22K 的 2 种主要蛋白质。 22K 种类被认为代表了主要的包膜多肽；在感染性培养基中，它存在于低密度的亚结构中，可以通过洗涤剂处理定量地转化为缓慢沉淀的材料。 20K多肽（占病毒体中14C氨基酸标记的大约80%）被磷酸化，发生在比病毒体密度更高的细胞内亚结构中（在CsCl中ρ = 1.36 g/ml），并且是唯一具有RNA结合特性的病毒蛋白；在免疫沉淀中它优先被异源动物血清识别。因此，20K 种被确定为主要的衣壳蛋白。在受感染细胞的提取物中定期检测到另外两种多肽（19K 和 17K）；它们似乎与 20K 蛋白共享寡肽，并被解释为蛋白水解裂解产物。伯尔尼病毒的核衣壳在乙醚处理后可视化为具有明显横向条纹的柔性杆状结构。 20K 核衣壳蛋白的证实进一步支持了伯尔尼病毒是包膜 RNA 病毒新家族（Toroviridae）代表的观点。
In Berne virsu-infected cells and in gradient-purified virions 2 major proteins with MW of 20,000 (20K) and 22K were detected. The 22K species is thought to represent the main envelope polypeptide; in infectious culture media it was present in a low density substructure which could be quantitatively converted into slowly sedimenting material by detergent treatment. The 20K polypeptide (accounting for .apprx. 80% of the 14C-amino acid label in the virion) was phosphorylated, occurred in an intracellular substructure of higher density than the virion (.rho. = 1.36 g/ml in CsCl) and was the only viral protein possessing RNA-binding properties; it was recognized preferentially by heterologous animal sera in immune precipitation. The 20K species is therefore identified as the main capsid protein. Two additional polypeptides (19K and 17K) were regularly detected in extracts of infected cells; they appeared to share oligopeptides with the 20K protein and are interpreted as being proteolytic cleavage products. The nucleocapsid of Berne virus was visualized after ether treatment as a flexible bacilliform structure with conspicuous transverse striation. Demonstration of a 20K nucleocapsid protein further supports the proposal that Berne virus is a representative of a new family of enveloped RNA viruses (Toroviridae).