Genome-wide approaches to determining origin distribution
Genome-wide approaches to determining origin distribution
复制标题
DOI：
10.1007/s10577-009-9094-2
复制
发表时间：
2010-01-01
影响因子：
2.6
通讯作者：
Prioleau, Marie-Noelle
中科院分区：
文献类型：
--
作者：
Cadoret, Jean-Charles;Prioleau, Marie-Noelle
基因组的完整性取决于一个高度协调的过程，确保基因组在每个细胞周期的精确复制。哺乳动物中DNA复制起点的基因组图谱，称为复制起点，是我们理解这种重要机制如何在整个复杂基因组中调节的重要一步。最近在人类和小鼠细胞中进行的两项研究揭示了复制起点和转录调控元件之间的强烈关联。这种强烈的重叠提出了一个问题，即基因沙漠，也缺乏复制起点，如何在复制中断的条件下正确复制。这也为进一步研究DNA复制起始的关键调控因子提供了有价值的信息.在这里，我们回顾了这些大规模的复制起点映射带来了我们的理解复制启动和未来的前景是什么。
Genome integrity depends upon a highly co-ordinated process that ensures the exact duplication of the genome at each cell cycle. Genomic mapping of DNA replication starting points in mammals, known as origins of replication, is an important step towards our understanding of how this essential mechanism is regulated throughout complex genomes. Two recent studies carried out in both human and mouse cells have revealed a strong association between replication origins and transcriptional regulatory elements. This strong overlap raises the question of how gene deserts, also lacking replication origins, are properly replicated in conditions where replication is disrupted. It also provides valuable information forward the identification of key regulatory factors of DNA replication initiation. Here, we review what these large-scale mappings of replication origins have brought to our understanding of replication initiation and what are the future prospects.