Mapping protein-RNA interactions by RCAP, RNA-cross-linking and peptide fingerprinting.
Mapping protein-RNA interactions by RCAP, RNA-cross-linking and peptide fingerprinting.
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通过 RCAP、RNA 交联和肽指纹图谱绘制蛋白质-RNA 相互作用。
DOI：
10.1007/978-1-4939-2562-9_16
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Kao,CCheng
中科院分区：
文献类型：
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作者：
Vaughan,RobertC;Kao,CCheng
RNA纳米技术通常以蛋白质RNA复合体为特征。蛋白质和大RNA之间的相互作用很难用核磁共振或X射线结晶学等传统的基于结构的方法来研究。RCAP是一种使用可逆交联亲和纯化方法与质谱学相结合的方法，已被开发用于定位与RNA接触的蛋白质区域。本章详细介绍了RCAP如何应用于绘制病毒粒子内的蛋白质-RNA接触图。
RNA nanotechnology often feature protein RNA complexes. The interaction between proteins and large RNAs are difficult to study using traditional structure-based methods like NMR or X-ray crystallography. RCAP, an approach that uses reversible-cross-linking affinity purification method coupled with mass spectrometry, has been developed to map regions within proteins that contact RNA. This chapter details how RCAP is applied to map protein–RNA contacts within virions.