Structure of the gene encoding mouse adipose differentiation-related protein (ADRP).
Structure of the gene encoding mouse adipose differentiation-related protein (ADRP).
复制标题
编码小鼠脂肪分化相关蛋白（ADRP）的基因的结构。
DOI：
10.1006/geno.1993.1241
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发表时间：
1993
期刊：
影响因子：
4.4
通讯作者：
Serrero,G
中科院分区：
文献类型：
--
作者：
Eisinger,DP;Serrero,G
脂肪分化相关蛋白（Adipose differentiation-related protein， ADRP）是一种新的50 kda的膜相关蛋白，其信息水平在触发脂肪细胞分化后被迅速和最大限度地诱导。从四个重叠的λ噬菌体克隆中分离并鉴定了编码小鼠ADRP的基因。该基因全长14kb，包含8个外显子和7个内含子。外显子的大小在50 - 696 bp之间，内含子的大小在87 - 4.3 kb之间。主要和次要转录起始位点分别位于引发剂蛋氨酸上游76和78 bp。TATTTTA序列集中在主要转录起始位点上游30 bp处，在5 '侧区有几个推测的转录因子结合位点。ADRP已被定位到4号染色体上，特别是在双镰带之间。第二个adrp样基因被分离并部分鉴定。第二个位点在12种不同的小鼠组织中不表达，在分析的外显子和内含子区域上与ADRP具有87%的序列相似性。最后，这是首次报道的ADRP的基因组结构。
Adipose differentiation-related protein (ADRP) is a novel 50-kDa membrane-associated protein whose message levels are induced rapidly and maximally after triggering adipocyte differentiation. The gene encoding mouse ADRP has been isolated and characterized from four overlapping λ phage clones. The gene spans 14 kb and contains 8 exons and 7 introns. Exons range in size from 50 to 696 bp and intron sizes range from 87 bp to 4.3 kb. Major and minor transcription initiation sites were determined 76 and 78 bp, respectively, upstream of the initiator methionine. A TATTTTA sequence is centered 30 bp upstream of the major transcription start site, and within the 5′-flanking region there are several putative transcription factor binding sites. ADRP has been mapped to chromosome 4, specifically between thebandIfaloci. A second ADRP-like gene was isolated and partially characterized. This second locus is not expressed in 12 different mouse tissues and shares 87% sequence similarity to ADRP over exon and intron regions analyzed. Finally, this is the first reported genomic structure of ADRP.