Dynamic Mechanical Loading Enhances Functional Properties of Tissue-Engineered Cartilage Using Mature Canine Chondrocytes
Dynamic Mechanical Loading Enhances Functional Properties of Tissue-Engineered Cartilage Using Mature Canine Chondrocytes
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DOI：
10.1089/ten.tea.2009.0482
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发表时间：
2010-05-01
影响因子：
4.1
通讯作者：
Hung, Clark T.
中科院分区：
文献类型：
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作者：
Bian, Liming;Fong, Jason V.;Hung, Clark T.
目的：软骨功能组织工程的概念促进了生理性负载生物反应器系统在培养具有承载性能的工程化组织中的应用。先前的研究已经证明，将未成熟关节的原代牛软骨细胞接种到琼脂糖构建物中，并经过动态变形，可以产生与天然组织相匹配的平衡压缩性能和蛋白多糖含量。在将这些结果转化为成年犬动物模型的过程中，人们发现，以前在未成熟的牛原代软骨细胞上成功的方案在使用成年犬原代软骨细胞时不会产生同样的成功结果。本研究的目的是评估在琼脂糖凝胶中种植的成年犬软骨细胞承受动态加载的改良FTE方案的有效性。方法：对使用定制生物反应器的构建物施加两种动态加载模式：无约束轴向压缩变形加载(DL；1 Hz，10%变形)或滑动接触加载(Slide，0.5 Hz，10%变形)。在培养的第0、14或28天(DL0、DL14、DL28和Slide14)开始每天加载3h。结果：在培养的第28天，施加加载(DL和Slide)的支架与自由肿胀对照组相比，杨氏模量显著增加(p
Objective: The concept of cartilage functional tissue engineering (FTE) has promoted the use of physiologic loading bioreactor systems to cultivate engineered tissues with load-bearing properties. Prior studies have demonstrated that culturing agarose constructs seeded with primary bovine chondrocytes from immature joints, and subjected to dynamic deformation, produced equilibrium compressive properties and proteoglycan content matching the native tissue. In the process of translating these results to an adult canine animal model, it was found that protocols previously successful with immature bovine primary chondrocytes did not produce the same successful outcome when using adult canine primary chondrocytes. The objective of this study was to assess the efficacy of a modified FTE protocol using adult canine chondrocytes seeded in agarose hydrogel and subjected to dynamic loading.Method: Two modes of dynamic loading were applied to constructs using custom bioreactors: unconfined axial compressive deformational loading (DL; 1 Hz, 10% deformation) or sliding contact loading (Slide; 0.5 Hz, 10% deformation). Loading for 3 h daily was initiated on day 0, 14, or 28 (DL0, DL14, DL28, and Slide14).Results: Constructs with applied loading (both DL and Slide) exhibited significant increases in Young's modulus compared with free-swelling control as early as day 28 in culture (p