Genetic incompatibilities in reciprocal hybrids between populations of Tigriopus californicus with low to moderate mitochondrial sequence divergence
Genetic incompatibilities in reciprocal hybrids between populations of Tigriopus californicus with low to moderate mitochondrial sequence divergence
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DOI：
10.1093/evolut/qpad122
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发表时间：
2023-07-05
期刊：
影响因子：
3.3
通讯作者：
Burton，Ronald S.
中科院分区：
文献类型：
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作者：
Healy，Timothy M.;Burton，Ronald S.
所有线粒体编码的蛋白质和RNA都通过与核编码的蛋白质相互作用来发挥作用，这对线粒体的性能和真核生物的适合性至关重要。共同进化维持分类单元内基因组间(即有丝分裂核)的亲和性，但杂交可以破坏共同进化的相互作用，导致杂交失败。因此，有丝分裂不亲和性可能是生殖隔离和潜在的物种形成的重要机制。在这里，我们利用Pool-Seq来评估线粒体基因对快速和缓慢发育的相互种群间F2代的核等位基因频率的影响。我们发现，在线粒体DNA核苷酸序列存在1.5%或9.6%固定差异的群体之间的杂交中，有丝分裂核相互作用导致两条染色体上共同进化(即母体)核等位基因的频率增加。然而，我们也发现了每个杂交分别在三条或四条染色体上存在过多错配(即非共同进化)等位基因的证据，以及等位基因频率差异与仅涉及核基因座(即不受线粒体基因影响)的影响一致的证据。因此，我们对低分歧杂交的结果表明，有丝分裂相互作用在杂交发育率的变异中起着潜在的作用，但尽管有丝分裂共同进化对单个染色体有实质性的影响，总体上没有明显的有利于共同进化相互作用的倾向。
All mitochondrial-encoded proteins and RNAs function through interactions with nuclear-encoded proteins, which are critical for mitochondrial performance and eukaryotic fitness. Coevolution maintains inter-genomic (i.e., mitonuclear) compatibility within a taxon, but hybridization can disrupt coevolved interactions, resulting in hybrid breakdown. Thus, mitonuclear incompatibilities may be important mechanisms underlying reproductive isolation and, potentially, speciation. Here we utilize Pool-seq to assess the effects of mitochondrial genotype on nuclear allele frequencies in fast- and slow-developing reciprocal inter-population F2hybrids between relatively low-divergence populations of the intertidal copepodTigriopus californicus. We show that mitonuclear interactions lead to elevated frequencies of coevolved (i.e., maternal) nuclear alleles on two chromosomes in crosses between populations with 1.5% or 9.6% fixed differences in mitochondrial DNA nucleotide sequence. However, we also find evidence of excess mismatched (i.e., noncoevolved) alleles on three or four chromosomes per cross, respectively, and of allele frequency differences consistent with effects involving only nuclear loci (i.e., unaffected by mitochondrial genotype). Thus, our results for low-divergence crosses suggest an underlying role for mitonuclear interactions in variation in hybrid developmental rate, but despite substantial effects of mitonuclear coevolution on individual chromosomes, no clear bias favoring coevolved interactions overall.