Role of osteopontin in bone remodeling caused by mechanical stress
Role of osteopontin in bone remodeling caused by mechanical stress
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DOI：
10.1359/jbmr.1999.14.6.839
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发表时间：
1999-06-01
影响因子：
6.2
通讯作者：
Nomura, S
中科院分区：
文献类型：
--
作者：
Terai, K;Takano-Yamamoto, T;Nomura, S
在本研究中，骨桥蛋白mRNA（Opn）表达的骨细胞和破骨细胞的数量和比例的变化所造成的机械应力施加在实验性牙齿移动。OPN表达在早期的压力侧骨细胞中，并逐渐扩散到张力侧的骨细胞以及牙槽骨中的成骨细胞和骨衬里细胞。在对照组大鼠的根间隔膜中，仅3.3%的压力侧骨细胞表达Opn，而在牙齿移动开始后48 h，该值增加到87.5%。这些结果表明，这些细胞响应于骨上加载的机械应力，表达骨桥蛋白基因。在这些细胞中Opn表达增加后，与对照组相比，破骨细胞的数量增加了17倍，在牙槽骨的压力侧观察到许多吸收坑。注射精氨酸-甘氨酸-天冬氨酸-丝氨酸肽而不是精氨酸-甘氨酸-谷氨酸-丝氨酸肽可强烈抑制破骨细胞数量的增加。体外迁移实验证实骨桥蛋白（OPN）对破骨细胞前体具有趋化活性。我们的研究有力地表明，OPN是一个重要的因素触发骨重建所造成的机械应力。
Changes in the number and proportion of osteopontin mRNA (Opn) expressing osteocytes and osteoclasts caused by the mechanical stress applied during experimental tooth movement were examined in the present study. Opn expression was detected in the osteocytes on the pressure side at the early stage, and gradually spread to those on the tension side and also to the osteoblasts and bone-lining cells in the alveolar bone. Only 3.3% of the osteocytes located on the pressure side expressed Opn in the interradicular septum of control rats; in contrast, the value was increased to 87.5 % at 48 h after the initiation of tooth movement. These results indicate that these cells responded to mechanical stress loaded on the bone with expression of the osteopontin gene. Following the increased expression of Opn in these cells, a 17-fold greater number of osteoclasts compared with the control and numerous resorption pits were observed on the pressure side of the alveolar bone. Injection of arginine-glycine-aspartic acid-serine peptide but not that of arginine-glycine-glutamic acid-serine peptide strongly inhibited the increase in the number of osteoclasts, Furthermore, an in vitro migration assay demonstrated the chemotactic activity of osteopontin (OPN) on the precursor of osteoclasts. Our study strongly suggests that OPN is an important factor triggering bone remodeling caused by mechanical stress.