Drosophila p24 and Sec22 regulate Wingless trafficking in the early secretory pathway.
Drosophila p24 and Sec22 regulate Wingless trafficking in the early secretory pathway.
复制标题
果蝇 p24 和 Sec22 调节早期分泌途径中的 Wingless 运输
DOI：
10.1016/j.bbrc.2015.04.151
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发表时间：
2015-08-07
影响因子：
3.1
通讯作者：
Lin X
中科院分区：
文献类型：
--
作者：
Li X;Wu Y;Shen C;Belenkaya TY;Ray L;Lin X
Wnt信号通路对发育和疾病至关重要。Wnt蛋白转运的调控是Wnt研究领域的关键问题之一。在这里，我们在果蝇中进行了参与Wingless/Wnt分泌的基因的遗传筛选，并确定了p24蛋白家族成员Baiser，CHOp 24，Eclair和v-SNARE蛋白Sec 22，它们参与Wingless/Wnt的早期分泌途径。我们提供的遗传证据表明，p24蛋白或Sec 22的损失阻碍无翅（Wg）分泌果蝇翅成虫盘。我们发现Baiser不能替代其他p24蛋白（CHOp 24或Eclair）护送Wg，只有Baiser和CHOp 24与Wg相互作用。此外，我们发现v-SNARE蛋白Sec 22和Wg与p24蛋白包装在一起。总之，我们的数据提供了重要的见解Wg/Wnt的早期分泌途径。
The Wnt signaling pathway is crucial for development and disease. The regulation of Wnt protein trafficking is one of the pivotal issues in the Wnt research field. Here we performed a genetic screen in Drosophila Melanogaster for genes involved in Wingless/Wnt secretion, and identified the p24 protein family members Baiser, CHOp24, Eclair and a v-SNARE protein Sec22, which are involved in the early secretory pathway of Wingless/Wnt. We provided genetic evidence demonstrating that loss of p24 proteins or Sec22 impedes Wingless (Wg) secretion in Drosophila wing imaginal discs. We found that Baiser cannot replace other p24 proteins (CHOp24 or Eclair) in escorting Wg, and only Baiser and CHOp24 interact with Wg. Moreover, we showed that the v-SNARE protein Sec22 and Wg are packaged together with p24 proteins. Taken together, our data provide important insights into the early secretory pathway of Wg/Wnt.