In vivo evaluation of bone-bonding ability of RGD-coated porous implant using layer-by-layer electrostatic self-assembly.
In vivo evaluation of bone-bonding ability of RGD-coated porous implant using layer-by-layer electrostatic self-assembly.
复制标题
DOI：
10.1002/jbm.a.32055
复制
发表时间：
2009-06
期刊：
影响因子：
--
通讯作者：
Guo-li Yang;F. He;Xiao-feng Yang;Xiao-xiang Wang;Shi-fang Zhao
中科院分区：
文献类型：
--
作者：
Guo-li Yang;F. He;Xiao-feng Yang;Xiao-xiang Wang;Shi-fang Zhao
RGD已被证明可以改善种植体的骨结合。然而，关于RGD涂层对螺纹状多孔种植体的骨结合能力的影响的研究很少。本研究的目的是研究采用逐层自组装技术的RGD涂层对多孔种植体的骨结合能力的影响。30只兔股骨植入60枚长度为10 mm的种植体(对照组30枚，RGD涂层30枚)；15只兔胫骨植入30枚8 mm长的种植体(对照组15枚，RGD涂层15枚)。分别于植入后4、8、12周取出股骨和胫骨，进行扭矩去除试验(RTQ)和组织形态计量学评价。术后4周，两种种植体的RTQ值差异无统计学意义(p=0.932)。8周和12周的RTQ值比较，差异有统计学意义(p=0.002，0.001)。两种种植体表面均有新骨形成。骨-种植体接触模式似乎在两个种植体中产生了广泛的直接接触。在第4、8和12周，涂有RGD涂层的种植体在皮质骨内的骨线中的BIC显著高于对照种植体(p分别为0.024、0.041和0.022)。4周时，两种种植体在同一螺纹内的骨面积无差异(p=0.806)，而在8周和12周时，两种种植体之间存在差异(p=0.009，0.031)。由此可见，采用层层自组装技术的RGD涂层对多孔种植体的骨结合能力有积极的影响。
RGD has been demonstrated to improve implant osseointegration. However, few studies are known about an effect of RGD coating on a bone-bonding ability of screw-shaped porous implant. The aim of this study was to investigate the effect of RGD coating using the layer-by-layer self-assembly technique on the bone-bonding ability of porous implant. 60 implants of 10 mm in length (30 control and 30 RGD-coated) were inserted into femurs of 30 rabbits and 30 implants of 8 mm in length (15 control and 15 RGD-coated) were inserted into tibias of 15 rabbits. At 4, 8, and 12 weeks post-implantation, femurs and tibias were retrieved and prepared for removal torque tests (RTQ) and histomorphometric evaluation, respectively. No differences were found in the RTQ values between two implants at 4 weeks (p = 0.932). There were statistical significances in the RTQ values at 8 and 12 weeks (p = 0.002, 0.001, respectively). New bone was formed on both implant surfaces. The bone-implant contact pattern appeared to produce a broad-based direct contact in both implants. The RGD-coated implants showed a significantly greater BIC in the threads inside the cortical bone compared with the control implants at 4, 8, and 12 weeks (p = 0.024, 0.041, 0.022, respectively). No differences were found in the bone area within the same threads between two implants at 4 weeks (p = 0.806) whereas differences were found at 8 and 12 weeks (p = 0.009, 0.031, respectively). It was concluded that RGD coating using the layer-by-layer self-assembly technique has a positive effect on the bone-bonding ability of porous implant.