Spatial relationships between fibroblasts during the growth of rat-tail tendon
Spatial relationships between fibroblasts during the growth of rat-tail tendon
复制标题
大鼠尾腱生长过程中成纤维细胞之间的空间关系
DOI：
10.1007/bf00217399
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发表时间：
2004
影响因子：
3.6
通讯作者：
Craig Magnes
中科院分区：
文献类型：
--
作者：
C. Squier;Craig Magnes
从出生后第18天至第244天，分8个时间间隔取大鼠尾根部肌腱切片，进行电镜观察。对于每个时间段，测量成纤维细胞、胶原蛋白和间质材料的相对面积、肌腱每单位面积的成纤维细胞数量和单个成纤维细胞的平均面积。采用“最近邻”法定量描述成纤维细胞在肌腱切片中的空间排列。最初，胶原蛋白的面积迅速增加，伴随着成纤维细胞所占面积的减少，但在104天的年龄后，这些值变化很小。从胚胎到104天，每单位面积的成纤维细胞数量稳步下降，而每个细胞的平均大小增加，在40天龄时达到最大面积，然后下降。在所有的时间间隔细胞排列在一个规则的，分散的模式横跨肌腱束。大鼠尾巴宽度的增长似乎涉及胶原蛋白和其他细胞间物质的分泌，对称地围绕每个成纤维细胞，以便逐渐分离细胞，直到达到一个阶段，在该阶段，细胞相距足够远，相邻的细胞突起之间几乎没有接触。这可能会干扰组织中代谢活动的整合。结果，细胞体收缩，分泌活动减少，因此，在55至104日龄之间，肌腱进入终末衰老期。
SummarySections of tendons from the base of the tail of rats were taken at eight time intervals from 18 days in utero until 244 days after birth and were examined in the electron microscope. For each time period, measurements were made of the relative area of fibroblasts, collagen and interstitial material, of the number of fibroblasts per unit area of tendon and of the average area of individual fibroblasts. The spatial arrangement of fibroblasts in the tendon sections was described quantitatively using the “nearest neighbor” method. Initially there was a rapid increase in the area of collagen accompanied by a decrease in the area occupied by fibroblasts but after 104 days of age these values changed very little. The numbers of fibroblasts per unit area decreased steadily from the embryo until 104 days whereas the average size of each cell increased to reach a maximum area at 40 days of age and then declined. At all time intervals cells were arranged in a regular, dispersed pattern across the tendon fascicles. Growth in width of the rat tail appears to involve the secretion of collagen and other intercellular material symmetrically around each fibroblast, so as to gradually separate the cells until a stage is reached at which cells are sufficiently far apart that there is little contact between adjacent cell processes. This may interfere with the integration of metabolic activity in the tissue. As a consequence, there is shrinkage of the cell bodies and a reduction in secretory activity so that, between 55 and 104 days of age, the tendon enters a period of terminal senescence.