Pseudomonas aeruginosa surface motility and invasion into competing communities enhances interspecies antagonism.
Pseudomonas aeruginosa surface motility and invasion into competing communities enhances interspecies antagonism.
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铜绿假单胞菌的表面运动和入侵竞争群落增强了种间拮抗作用。
DOI：
10.1101/2024.04.03.588010
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Limoli,DominiqueH
中科院分区：
文献类型：
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作者：
Sánchez-Peña,Andrea;Winans,JamesB;Nadell,CareyD;Limoli,DominiqueH
涉及铜绿假单胞菌和金黄色葡萄球菌的慢性多种微生物感染普遍存在，难以根除，并与不良健康结局相关。因此，了解这些病原体之间的相互作用对于改进治疗开发非常重要。我们以前证明了P. aerodynosais吸引到S. aureusing IV型皮利（TFP）介导的趋化性，但吸引对S. aureus生长和生理学仍然未知。使用活的单细胞共聚焦成像可视化微菌落结构，空间组织，和生存的S。aureus在共培养过程中，我们发现种间趋化性提供了P. aerodynamics通过促进入侵和破坏S.金色小菌落。此行为呈现S.金色感虫P.需氧抗生素相反，在缺乏TFP运动性的情况下，铜绿假单胞菌细胞表现出对S.金色菌落相反，铜绿假单胞菌在邻近S.金黄色葡萄球菌并分泌可扩散的抑菌抗菌剂如2-庚基-4-羟基喹啉-N-氧化物（HQNO）进入S.金色菌落减少侵入导致形成更致密和更厚的S。金黄色葡萄球菌菌落增加HQNO介导的乳酸发酵，生理变化，可能复杂的治疗策略。最后，我们证明了P.空间结构的好氧性改变增强了竞争力。金黄色。总的来说，这些研究扩展了我们对howP的理解。aerodecosaTFP-mediated种间趋化性促进多微生物的相互作用，突出了空间定位在混合物种community.IMPORTANCEThe许多慢性感染的多微生物的性质，使其根除的重要性。特别是，从患有囊性纤维化和慢性伤口感染的人的气道中共同分离出铜绿假单胞菌和金黄色葡萄球菌是常见的，并且与严重的临床结果相关。这些病原体之间复杂的相互作用尚未完全了解，这突出表明需要继续研究以改善慢性感染的管理。我们的研究揭示了P. aerodynosais吸引到S.金黄色葡萄球菌，入侵邻近菌落，并将抗葡萄球菌因子分泌到殖民地内部。抑制P.好氧性和入侵性，S. aureuscolony建筑变化剧烈，whereby S. aureusis对P.呼吸道拮抗作用，并通过可能进一步妨碍治疗的生理变化作出反应。这些研究加强了积累的证据，空间结构可以决定社区的弹性，并揭示了运动性和趋化性是种间竞争的关键驱动力。
Chronic polymicrobial infections involvingPseudomonas aeruginosaandStaphylococcus aureusare prevalent, difficult to eradicate, and associated with poor health outcomes. Therefore, understanding interactions between these pathogens is important to inform improved treatment development. We previously demonstrated thatP. aeruginosais attracted toS. aureususing type IV pili (TFP)-mediated chemotaxis, but the impact of attraction onS. aureusgrowth and physiology remained unknown. Using live single-cell confocal imaging to visualize microcolony structure, spatial organization, and survival ofS. aureusduring coculture, we found that interspecies chemotaxis providesP. aeruginosaa competitive advantage by promoting invasion into and disruption ofS. aureusmicrocolonies. This behavior rendersS. aureussusceptible toP. aeruginosaantimicrobials. Conversely, in the absence of TFP motility,P. aeruginosacells exhibit reduced invasion ofS. aureuscolonies. Instead,P. aeruginosabuilds a cellular barrier adjacent toS. aureusand secretes diffusible, bacteriostatic antimicrobials like 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO) into theS. aureuscolonies. Reduced invasion leads to the formation of denser and thickerS. aureuscolonies with increased HQNO-mediated lactic acid fermentation, a physiological change that could complicate treatment strategies. Finally, we show thatP. aeruginosamotility modifications of spatial structure enhance competition againstS. aureus. Overall, these studies expand our understanding of howP. aeruginosaTFP-mediated interspecies chemotaxis facilitates polymicrobial interactions, highlighting the importance of spatial positioning in mixed-species communities.IMPORTANCEThe polymicrobial nature of many chronic infections makes their eradication challenging. Particularly, coisolation ofPseudomonas aeruginosaandStaphylococcus aureusfrom airways of people with cystic fibrosis and chronic wound infections is common and associated with severe clinical outcomes. The complex interplay between these pathogens is not fully understood, highlighting the need for continued research to improve management of chronic infections. Our study unveils thatP. aeruginosais attracted toS. aureus, invades into neighboring colonies, and secretes anti-staphylococcal factors into the interior of the colony. Upon inhibition ofP. aeruginosamotility and thus invasion,S. aureuscolony architecture changes dramatically, wherebyS. aureusis protected fromP. aeruginosaantagonism and responds through physiological alterations that may further hamper treatment. These studies reinforce accumulating evidence that spatial structuring can dictate community resilience and reveal that motility and chemotaxis are critical drivers of interspecies competition.