Effect of graphene oxide on undifferentiated and retinoic acid-differentiated SH-SY5Y cells line
Effect of graphene oxide on undifferentiated and retinoic acid-differentiated SH-SY5Y cells line
复制标题
氧化石墨烯对未分化和视黄酸分化的SH-SY5Y细胞系的影响
DOI：
10.1039/c2nr30407d
复制
发表时间：
2012-01-01
期刊：
影响因子：
6.7
通讯作者：
Huang, Qing
中科院分区：
文献类型：
--
作者：
Lv, Min;Zhang, Yujie;Huang, Qing
氧化石墨烯（GO）因其丰富的官能团和良好的水溶性，为生物学领域的开发和应用创造了前所未有的机遇。近年来，GO在环境和人体中的潜在毒性引起了越来越多的关注。在本文中，我们通过检测GO对人神经母细胞瘤SH-SY5Y细胞系形态、活力和分化的影响，系统地研究了GO纳米片的细胞毒性，该细胞系是体外研究神经元疾病的理想模型。结果表明GO在低浓度(=80μg·mL(-1))下无明显细胞毒性。此外，GO不诱导细胞凋亡。非常有趣的是，GO 通过评估神经突长度和神经元标记物 MAP2 的表达，显着增强了 SH-SY5Y 诱导的视黄酸 (RA) 的分化。这些数据为神经退行性疾病提供了有前景的应用。
Graphene oxide (GO), has created an unprecedented opportunity for development and application in biology, due to its abundant functional groups and well water solubility. Recently, the potential toxicity of GO in the environment and in humans has garnered more and more attention. In this paper, we systematically studied the cytotoxicity of GO nanosheets via examining the effect of GO on the morphology, viability and differentiation of a human neuroblastoma SH-SY5Y cell line, which was an ideal model used to study neuronal disease in vitro. The results suggested that GO had no obvious cytotoxicity at low concentration (= 80 mu g mL(-1)). Moreover, GO did not induce apoptosis. Very interestingly, GO significantly enhanced the differentiation of SH-SY5Y induced-retinoic acid (RA) by evaluating neurite length and the expression of neuronal marker MAP2. These data provide a promising application for neurodegenerative diseases.