Ambulatory ECG recording in mice.
Ambulatory ECG recording in mice.
复制标题
DOI：
10.3791/1739
复制
发表时间：
2010-05-27
期刊：
影响因子：
--
通讯作者：
Wehrens, Xander H T
中科院分区：
文献类型：
--
作者：
McCauley, Mark D;Wehrens, Xander H T
小鼠遥测心电图记录对于了解心律失常、传导障碍和心源性猝死背后的机制至关重要。尽管表面心电图用于波形间隔的短期测量，但对于心率变异性的长期研究或捕获罕见的心律失常发作并不实用。植入式心电图遥测仪具有手术植入简单、可长期记录活动小鼠心电图以及可同时记录多只动物的可扩展性等优点。在这里，我们提出了植入遥测仪以记录小鼠动态心电图的分步指南。为了获得良好的生存结果，需要仔细遵守无菌技术，并有可能进行数周至数月的植入和记录。因此，植入式心电图遥测技术是一种有价值的工具，可用于检测小鼠等动态动物模型中心脏电生理学的关键信息。
Telemetric ECG recording in mice is essential to understanding the mechanisms behind arrhythmias, conduction disorders, and sudden cardiac death. Although the surface ECG is utilized for short-term measurements of waveform intervals, it is not practical for long-term studies of heart rate variability or the capture of rare episodes of arrhythmias. Implantable ECG telemeters offer the advantages of simple surgical implantation, long-term recording of electrograms in ambulatory mice, and scalability with simultaneous recordings of multiple animals. Here, we present a step-by-step guide to the implantation of telemeters for ambulatory ECG recording in mice. Careful adherence to aseptic technique is required for favorable survival results with the possibility of implantation and recording from weeks to months. Thus, implantable ECG telemetry is a valuable tool for detection of critical information on cardiac electrophysiology in ambulatory animal models such as the mouse.