Histamine-treated dendritic cells improve recruitment of type 2 CD8 T cells in the lungs of allergic mice
Histamine-treated dendritic cells improve recruitment of type 2 CD8 T cells in the lungs of allergic mice
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DOI：
10.1111/j.1365-2567.2010.03262.x
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发表时间：
2010-08-01
期刊：
影响因子：
6.4
通讯作者：
Vermeulen, Monica
中科院分区：
文献类型：
--
作者：
Amaral, Maria M.;Alvarez, Carolina;Vermeulen, Monica
组胺对树突状细胞（DCs）的功能有调节作用.这似乎是发展中国家正常发展所必需的。它还诱导未成熟DC的趋化性，并促进CD 4 + T细胞分化为具有辅助性T细胞2型（Th 2）特征的细胞。此外，我们最近发现组胺刺激DC对抗原的摄取和交叉呈递，这支持了组胺在过敏性病理发展过程中促进CD 8 + T细胞活化的理论。在这里，我们研究了是否过敏反应的过程中，在一个明确的模型卵清蛋白（OVA）诱导的过敏性气道炎症，可以调制的卵白蛋白脉冲树突状细胞（DCHISs）预先治疗的组胺（DCHISs）intrichheal注射。与对照DC相比，DCHIS诱导：（i）肺中CD 8 + T细胞的更多募集，（ii）肺CD 8 + T细胞产生白细胞介素（IL）-5的更大刺激，以及（iii）过敏小鼠肺中CD 11 c/CD 8双阳性DC的募集增加。此外，DCHIS处理的小鼠显示针对OVA的血清特异性免疫球蛋白E（IgE）抗体水平增加，支气管肺泡灌洗液（BAL）中嗜酸性粒细胞的比例较高，与用OVA脉冲的对照DC处理的小鼠相比。我们的研究结果支持这样的观点，即组胺，通过作用于树突状细胞，增加过敏过程的严重程度。
P>Histamine controls the function of dendritic cells (DCs). It appears to be required for the normal development of DCs. It also induces the chemotaxis of immature DCs and promotes the differentiation of CD4+ T cells into cells with a T helper type 2 (Th2) profile. Moreover, we have recently shown that histamine stimulates both the uptake and the cross-presentation of antigens by DCs, supporting the theory that histamine promotes activation of CD8+ T cells during the development of allergic pathologies. Here, we investigated whether the course of an allergic response, in a well-defined model of ovalbumin (OVA)-induced allergic airway inflammation, could be modulated by intratracheal injection of OVA-pulsed DCs previously treated with histamine (DCHISs). Compared with control DCs, DCHISs induced: (i) greater recruitment of CD8+ T cells in the lung, (ii) greater stimulation of the production of interleukin (IL)-5 by lung CD8+ T cells, and (iii) increased recruitment of CD11c/CD8 double-positive DCs in the lungs of allergic mice. Moreover, mice treated with DCHISs showed increased levels of serum-specific immunoglobulin E (IgE) antibodies directed to OVA, and a higher proportion of eosinophils in bronchoalveolar lavage (BAL) compared with mice treated with OVA-pulsed control DCs. Our results support the notion that histamine, by acting on DCs, increases the severity of allergic processes.