TOXICITY OF SHORT-TERM COPPER EXPOSURE TO EARLY LIFE STAGES OF RED SEA BREAM, PAGRUS MAJOR
TOXICITY OF SHORT-TERM COPPER EXPOSURE TO EARLY LIFE STAGES OF RED SEA BREAM, PAGRUS MAJOR
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DOI：
10.1002/etc.247
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发表时间：
2010-09-01
影响因子：
4.1
通讯作者：
Dou, Shuozeng
中科院分区：
文献类型：
--
作者：
Cao, Liang;Huang, Wei;Dou, Shuozeng
对红鲷（Pagrus major）胚胎和幼鱼进行了急性（0、0.1、0.2、0.4、0.8、1.6 mg Cu/L）和慢性（0、0.02、0.04、0.06、0.08、0.10、0.12 mg Cu/L）铜毒性试验，研究了它们在18 +/- 1℃静态水中（溶解有机碳1.8 +/- 0.65 mg C/L；硬度6183 +/- 360 mg CaCO3/L；盐度33 + 1‰）对铜的生物反应。24 h和48 h的LC50分别为0.23和0.15 mg/L，而48 h、72 h和96 h的LC50分别为0.52、0.19和0.13 mg/L，表明胚胎对铜的毒性比幼虫更敏感。当铜浓度为>= 0.06 mg/L时，幼虫的孵化成功率低，胚胎的孵化时间延迟，幼虫的生长和蛋黄吸收减少，而当铜浓度为>= 0.08 mg/L时，胚胎和幼虫的死亡率和形态畸形发生率均较高。铜浓度对红鲷鱼胚胎心率无显著影响，但当铜浓度为>= 0.08 mg/L时，可显著降低新孵化仔鱼的心率，说明高浓度铜在红鲷鱼幼体期比胚胎期更容易引起心跳紊乱。孵化成功率、孵化时间、生长速度、形态异常、卵黄吸收和心率均与铜浓度有关，可作为评价天然红鲷早期铜毒性的有效终点。环绕。Toxicol。化学2010;29:2044 - 2052。(c) 2010年第6段
Acute (0, 0.1, 0.2, 0.4, 0.8, 1.6 mg Cu/L) and chronic (0, 0.02, 0.04, 0.06, 0.08, 0.10, 0.12 mg Cu/L) toxicity tests of Cu with embryonic and larval red sea bream, Pagrus major, were carried out to investigate their biological responses to Cu exposure in static water at 18 +/- 1 degrees C (dissolved organic carbon, 1.8 +/- 0.65 mg C/L; hardness, 6,183 +/- 360 mg CaCO3/L; salinity, 33 + 1 parts per thousand). The 24- and 48-h LC50 (median lethal concentration) values of Cu for embryos were 0.23 and 0.15 mg/L, whereas the 48-, 72-, and 96-h LC50 values for larvae were 0.52, 0.19, and 0.13 mg/L, respectively, suggesting that embryos were more sensitive to Cu toxicity than larvae. Copper exposures at >= 0.06 mg concentrations caused low hatching success, a delay in the time to hatching of embryos, and reductions in the growth and yolk absorption of the larvae, whereas high mortality and morphological malformations occurred in the embryos and larvae at >= 0.08 mg/L concentrations. Copper concentration did not significantly affect the heart rate of the embryos, but it significantly decreased the heart rate of the newly hatched larvae when the Cu concentration was >= 0.08 mg/L, suggesting that Cu at high concentrations could induce heartbeat disturbances in red sea bream more easily at the larval stage than at the embryonic stage. Hatching success, time to hatching, growth rate, morphological abnormality, yolk absorption, and heart rate were Cu concentration-dependent and could be effective endpoints for evaluating Cu toxicity to the early life stages of red sea bream in nature. Environ. Toxicol. Chem. 2010;29:2044-2052. (C) 2010 SETAC