Molecular epidemiology of malaria in Cameroon.: XI.: Geographic distribution of Plasmodium falciparum isolates with dihydrofolate reductase gene mutations in southern and central Cameroon
Molecular epidemiology of malaria in Cameroon.: XI.: Geographic distribution of Plasmodium falciparum isolates with dihydrofolate reductase gene mutations in southern and central Cameroon
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DOI：
10.4269/ajtmh.2002.67.378
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发表时间：
2002-10-01
影响因子：
3.3
通讯作者：
Soula, G
中科院分区：
文献类型：
--
作者：
Basco, LK;Ndounga, M;Soula, G
二氢叶酸还原酶（dhfr）基因的DNA序列，乙胺嘧啶抗性的分子标记，确定了178个恶性疟原虫现场分离株收集沿着东西轴在喀麦隆南部。具有野生型dhfr等位基因的分离株比例从东部（贝尔图阿市）的48.1%到中部省份（雅温得和埃塞卡）的11.3-15.7%和沿岸的地区（港口城市杜阿拉）的0%不等。具有单个Asn-108突变或双突变（Ile-51或Arg-59和Asn-108）的分离株约占样本的10%。在东部（贝尔图阿），具有三重突变（Ile-51、Arg-59和Asn-108）的分离株与野生型分离株的比例相等（48.1%），而三重突变在雅温得（62.3%）、埃塞卡（62.7%）和杜阿拉（78.9%）占主导地位。三重dhfr突变沿着喀麦隆南部东西轴线的分布表明，从西部沿海地区到中部地区，然后向东到该国内地，存在一个递减的梯度。
The DNA sequence of the dihydrofolate reductase (dhfr) gene, a molecular marker for pyrimethamine resistance, was determined for 178 field isolates of Plasmodium falciparum collected along the east-west axis in southern Cameroon. The proportion of isolates having the wild-type dhfr allele varied from 48.1% in the east (city of Bertoua) to 11.3-15.7% in central provinces (Yaounde and Eseka) and 0% in the littoral region (port city of Douala). Isolates with a single Asn-108 mutation or double mutations (Ile-51 or Arg-59 and Asn-108) constituted approximately 10% of the samples. Isolates with triple mutations (Ile-51, Arg-59, and Asn-108) were present in an equal proportion (48.1%) as the wild-type isolates in the east (Bertoua), while triple mutations predominated in Yaounde (62.3%), Eseka (62.7%), and Douala (78.9%). The distribution of triple dhfr mutations along the east-west axis in southern Cameroon suggests the presence of a decreasing gradient from the west coastal region to the central region and then to the east towards the interior of the country.