Improvement of seed yields under boron-limiting conditions through overexpression of BOR1, a boron transporter for xylem loading, in Arabidopsis thaliana
Improvement of seed yields under boron-limiting conditions through overexpression of BOR1, a boron transporter for xylem loading, in Arabidopsis thaliana
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DOI：
10.1111/j.1365-313x.2006.02763.x
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发表时间：
2006-06-01
期刊：
影响因子：
7.2
通讯作者：
Fujiwara, Toru
中科院分区：
文献类型：
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作者：
Miwa, Kyoko;Takano, Junpei;Fujiwara, Toru
土壤施肥是现代农业中的常见做法，用于防止作物营养缺乏。然而，施肥成本高，并造成环境污染。种植耐低养分供应的植物可以避免这个问题。在这里，我们报告的一代拟南芥植物，耐受硼（B）缺乏的条件下，由于BOR 1，一个外排B转运蛋白，需要有效的木质部加载的B的过表达。在花椰菜花叶病毒35 S RNA启动子控制下表达BOR 1或BOR 1-GFP的几个独立产生的转基因植物中，与野生型植物相比，在B限制条件下，B的根到茎的易位得到增强，并且茎生长更大。此外，转基因植株表现出增加的B易位，特别是向茎尖，并在B限制条件下正常结实，在此条件下野生型植株不能结实。因此，本研究报告的植物，表现出改善种子产量相比，野生型在营养缺乏的条件下，作为一个重要的矿物质营养转运蛋白的生产增加的结果。
Soil fertilization is a common practice in modern agriculture, undertaken to prevent nutrient deficiency in crops. However, fertilization is costly and causes environmental pollution. The cultivation of plants that tolerate low nutrient supplies may circumvent this problem. Here, we report the generation of Arabidopsis thaliana plants that tolerate boron (B)-deficient conditions due to the overexpression of BOR1, an efflux B transporter that is required for efficient xylem loading of B. In several independently generated transgenic plants expressing BOR1 or BOR1-GFP under the control of the cauliflower mosaic virus 35S RNA promoter, root-to-shoot translocation of B was enhanced and shoot growth was greater under B-limiting conditions compared with wild-type plants. In addition, the transgenic plants showed increased translocation of B, especially to the shoot apex, and set seed normally under B-limiting conditions, under which wild-type plants failed to set seed. This study therefore reports plants that show improved seed yields compared with wild-type under nutrient-deficient conditions as a result of increased production of an essential mineral nutrient transporter.