Characterisation of the pvdE gene which is required for pyoverdine synthesis in Pseudomonas aeruginosa
Characterisation of the pvdE gene which is required for pyoverdine synthesis in Pseudomonas aeruginosa
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DOI：
10.1016/0378-1119(96)00209-0
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发表时间：
1996-10-17
期刊：
影响因子：
3.5
通讯作者：
Lamont, IL
中科院分区：
文献类型：
--
作者：
McMorran, BJ;Merriman, ME;Lamont, IL
铜绿假单胞菌（Pa）菌株PAO在缺铁条件下生长时合成铁载体pyoverdine （Pvd）。Pvd由一个二羟基喹啉基团连接到一个8-氨基酸残基肽。Pvd合成需要跨越至少78 kb染色体的DNA，但迄今为止，只有三个参与该过程的基因被表征。我们报告了第四个Pa基因的特征，pvdE，我们证明了Pvd合成所必需的。推断出的PvdE的氨基酸序列表明该蛋白是atp结合盒（ABC）膜转运蛋白家族的成员，这是ABC型蛋白参与铁载体合成的第一个例子。
Pseudomonas aeruginosa (Pa) strain PAO synthesises a siderophore, pyoverdine (Pvd), when grown under conditions of iron starvation. Pvd consists of a dihydroxyquinoline group attached to an 8-amino-acid-residue peptide. DNA spanning at least 78 kb of the chromosome is required for Pvd synthesis, but to date only three genes involved in this process have been characterised. We report the characterisation of a fourth Pa gene, pvdE, which we show to be required for Pvd synthesis. The deduced amino acid sequence of PvdE indicates that the protein is a member of the ATP-binding-cassette (ABC) family of membrane transporter proteins, and this is the first example of the involvement of an ABC-type protein in siderophore synthesis.