Chronic vitamin A intake affects the expression of mRNA for apolipoprotein A-I, but not for nuclear retinoid receptors, in liver of young and aging Lewis rats.
Chronic vitamin A intake affects the expression of mRNA for apolipoprotein A-I, but not for nuclear retinoid receptors, in liver of young and aging Lewis rats.
复制标题
长期摄入维生素 A 会影响年轻和老年 Lewis 大鼠肝脏中载脂蛋白 A-I mRNA 的表达，但不会影响核视黄醇受体的 mRNA 表达。
DOI：
10.1006/abbi.1995.9966
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发表时间：
1995
影响因子：
3.9
通讯作者：
Ross,AC
中科院分区：
文献类型：
--
作者：
Zolfaghari,R;Ross,AC
我们研究了慢性维甲酸状态和年龄对大鼠肝脏维甲酸反应基因载脂蛋白A-I（apo A-I）和三种核维甲酸受体RAR-α、RAR-β和RXR-α mRNAs稳态水平的影响。断奶的雄性刘易斯大鼠（每个年龄饮食组5只）喂食含维生素A的半合成饮食，维生素A处于边缘、对照或补充水平，直到它们2或8个月大。无论维生素A状态如何，老龄大鼠中载脂蛋白A-I mRNA的相对丰度（与β-肌动蛋白相比，β-肌动蛋白在治疗中没有差异）比年轻大鼠低约50%。对于两个年龄段，维生素A边缘组的apo A-I mRNA比对照组高约40-50%（P< 0.05），维生素A补充组的apo A-I mRNA比对照组低20-30%（P > 0.05）。慢性饮食和年龄都不影响肝脏中两种主要的维甲酸受体RAR-α或RXR-α的mRNA水平，也不影响已知由RA自身调节的受体RAR-β的mRNA水平。尽管维生素A对本研究中检测的受体的mRNA水平没有显著影响，但它们仍可能参与调节apo A-I基因表达，因为体外研究表明RAR-α和RXR-α均与apo A-I基因的调节区结合。
We have investigated the effects of chronic retinoid status and age on the steady-state levels in rat liver of the mRNAs for the retinoid-responsive gene apolipoprotein A-I (apo A-I), and for three nuclear retinoid receptors, RAR-α, RAR-β, and RXR-α. Weanling male Lewis rats (five per age–diet group) were fed a semisynthetic diet with vitamin A at marginal, control, or supplemented levels until they were 2 or 8 months of age. The relative abundance of apo A-I mRNA (compared to β-actin which did not differ among treatments) was about 50% lower in aging versus young rats, regardless of their vitamin A status. For both ages, apo A-I mRNA was approximately 40–50% higher (P< 0.05) for the vitamin A-marginal groups and 20–30% lower (P > 0.05) for the vitamin A-supplemented groups than the controls. Neither chronic diet nor age affected the levels of mRNA for RAR-α or RXR-α, two major retinoid receptors in liver, or for RAR-β, a receptor known to be autoregulated by RA. Although vitamin A did not have a significant effect on the mRNA levels of the receptors examined in this study, they are still likely to be involved in regulating apo A-I gene expression because in vitro studies have shown that both RAR-α and RXR-α bind to the regulatory region of the apo A-I gene.