Development and characterization of EST-SSR markers for Carex angustisquama (Cyperaceae), an extremophyte in solfatara fields
Development and characterization of EST-SSR markers for Carex angustisquama (Cyperaceae), an extremophyte in solfatara fields
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硫磺草田中的一种极端植物——狭叶苔草（莎草科）的 EST-SSR 标记的开发和表征
DOI：
10.1002/aps3.1185
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发表时间：
2018
影响因子：
3.6
通讯作者：
Sakaguchi Shota
中科院分区：
文献类型：
--
作者：
Nagasawa Koki;Setoguchi Hiroaki;Maki Masayuki;Goto Hayato;Fukushima Keitaro;Isagi Yuji;Sakaguchi Shota
本研究以日本北部北方盐沼地区特有的狭鳞苔草（Carexangustisquama）为研究对象，利用Illumina HiSeq 2000产生的RNA-Seq数据，开发了20个EST-SSR标记。在C. angustisquamaC. doenitziiandC.足妇科Inc. angustisquama群体内多个位点呈单态，平均等位基因数为1 ~ 5个，期望杂合度为0.000 ~ 0.580，而所有标记在一个群体内均呈多态性。邓尼茨。这表明，C. angustisquamais可能是由于该物种的种群动态，而不是无效等位基因在发达markers.ConclusionsThese标记将被用来评估遗传多样性和结构，并在未来的研究调查的进化历史的C。angustisquamaand related species.
Premise of the StudyExpressed sequence tag–simple sequence repeat (EST‐SSR) markers were developed forCarex angustisquama(Cyperaceae) to investigate the evolutionary history of this plant that is endemic to solfatara fields in northern Japan.Methods and ResultsUsing RNA‐Seq data generated by the Illumina HiSeq 2000, 20 EST‐SSR markers were developed. Polymorphisms were assessed inC. angustisquamaand the closely related speciesC. doenitziiandC. podogyna. InC. angustisquama, many loci were monomorphic within populations; the average number of alleles ranged from one to five, and levels of expected heterozygosity ranged from 0.000 to 0.580, while all markers were polymorphic in a population ofC. doenitzii. This indicates that low genetic polymorphism ofC. angustisquamais likely due to the species’ population dynamics, rather than to null alleles at the developed markers.ConclusionsThese markers will be used to assess genetic diversity and structure and to investigate evolutionary history in future studies ofC. angustisquamaand related species.