Application of antibody array technology in the analysis of urinary cytokine profiles in patients with chronic kidney disease
Application of antibody array technology in the analysis of urinary cytokine profiles in patients with chronic kidney disease
复制标题
抗体芯片技术在慢性肾脏病患者尿细胞因子谱分析中的应用
DOI：
10.1159/000096871
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发表时间：
2006-01-01
影响因子：
4.2
通讯作者：
Zhang, Xiao-liang
中科院分区：
文献类型：
--
作者：
Liu, Bi-Cheng;Zhang, Lu;Zhang, Xiao-liang
目的：新的证据表明，尿中细胞因子的排泄与慢性肾脏疾病（CKD）的进展有关。然而，高通量检测尿液中的细胞因子在临床实践中仍然是一个问题。在这项横断面研究中，我们应用了一种新的蛋白质组学技术，抗体阵列，分析CKD患者的尿液细胞因子谱。方法：10例受试者，包括7例CKD患者和3例正常对照者。根据估计肾小球滤过率（eGFR）水平将这些CKD患者分为两组：A组（eGFR ≥ 80 ml/min/1.73 m（2），n = 3）和B组（eGFR
Aims: Emerging evidence suggests that the urinary excretion of cytokines is associated with the progression of chronic kidney disease (CKD). However, detection of urinary cytokines in high throughput is still a problem in clinical practice. In this cross-sectional study, we applied a novel proteomic technology, antibody array, to analyze urinary cytokine profiles in patients with CKD. Methods: A total of 10 subjects including 7 CKD patients and 3 normal controls were studied. These patients with CKD were divided into two groups according to the levels of estimated glomerular filtration rate (eGFR): group A (eGFR >= 80 ml/min/1.73 m(2), n = 3) and group B (eGFR