Subunit interactions of the Escherichia coli mannitol permease: correlation with enzymic activities.
Subunit interactions of the Escherichia coli mannitol permease: correlation with enzymic activities.
复制标题
大肠杆菌甘露醇渗透酶的亚基相互作用：与酶活性的相关性。
DOI：
10.1021/bi00362a009
复制
发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Jacobson,GR
中科院分区：
文献类型：
--
作者：
Stephan,MM;Jacobson,GR
eiiml的材料及标记方法。含有编码甘露醇操纵子的质粒pLC15-48的大肠杆菌菌株MVI009的微型细胞被Leeet al.（1981）分离。根据Lee等人（1981）的描述，EIIMtl是通过用[35S]蛋氨酸孵育微型细胞，并加入1mm苯甲磺酰氟（PMSF）来抑制蛋白质水解来选择性标记的。然后用30 mM盐酸（tris -羟甲基）氨基甲烷盐酸（tris - hcl）， pH 8.0，
Materials and MethodsLabeling of EIIMtl. Minicellsof E. coli strain MVI009 containing the plasmid pLC15-48, which encodes the mannitol operon, were isolated as previously described by Leeet al.(1981). EIIMtl was selectively labeled by incubation of the minicells with [35S] methionine as described by Lee et al.(1981) with the inclusion of 1 mM phenylmethanesulfonyl fluoride (PMSF) to inhibit proteolysis. The minicells were then washed 3 times (12000g, 10 min) with 30 mM tris (hy-droxymethyl) aminomethane hydrochloride (Tris-HCl), pH 8.0,