Effects of peptide fraction and counter ion on the development of clinical signs in experimental autoimmune encephalomyelitis
Effects of peptide fraction and counter ion on the development of clinical signs in experimental autoimmune encephalomyelitis
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DOI：
10.1111/jnc.12664
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发表时间：
2014-05-01
影响因子：
4.7
通讯作者：
Feinstein, Douglas L.
中科院分区：
文献类型：
--
作者：
Boullerne, Anne I.;Polak, Paul E.;Feinstein, Douglas L.
最常用的诱导实验性自身免疫性脑脊髓炎（EAE）的免疫原是MOG（35-55），其是来源于髓鞘少突胶质细胞糖蛋白的21个残基的肽。MOG免疫方案的细微变化可以影响疾病的类型、平均发病日、疾病严重程度和总体发病率。我们推测，肽部分和抗衡离子的变化可能有助于这些差异。令人惊讶的是，用高（72%）肽级分的MOG制剂免疫导致比用仅具有45%肽级分的制剂免疫更低的平均EAE临床评分和更晚的疾病发作。用乙酸盐替代三氟乙酸盐（TFA）（用于MOG肽纯化的抗衡离子）延迟了疾病发作。这些结果表明，肽级分和存在的抗衡离子的知识与肽纯度一样重要，特别是当使用来自不同来源的肽时。图像显示TFA分子（未按比例）假设与MOG的带正电荷残基结合。
The most commonly used immunogen to induce experimental autoimmune encephalomyelitis (EAE) is MOG(35-55), a 21-residue peptide derived from myelin oligodendrocyte glycoprotein. Subtle variations in MOG immunization protocols can influence the type of disease, the average day of onset, disease severity, and overall incidence. We hypothesized that variance in peptide fraction and counter ion may contribute to those differences. Surprisingly, immunization with a MOG preparation of high (72%) peptide fraction resulted in lower average EAE clinical scores and later disease onset than immunization with a preparation having only 45% peptide fraction. Replacement of trifluoroacetate (TFA), the counter ion used for MOG peptide purification, with acetate delayed disease onset. These results show that knowledge of peptide fraction and the counter ion present are as critical as peptide purity, particularly when using peptides from different sources. The image shows TFA molecules (not to scale) hypothetically binding to the positively charged residues of MOG.